VAMP3 (8A3) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54366R-100uL |
| Product Name | VAMP3 (8A3) Monoclonal Antibody |
| Applications |
WB
IF(IHC-P)
IF(ICC)
IHC-P
FCM
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Synthetic peptide conjugated to KLH within N-terminal human VAMP3/Cellubrevin. |
| Clonality | Monoclonal |
| Clone # | 8A3 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Target | |
| Gene ID | 9341 |
| Swiss Prot | Q15836 |
| Subcellular location | Cytoplasm, Membrane bound |
| Synonyms | Vesicle-associated membrane protein 3; Cellubrevin; Synaptobrevin-3; VAMP-3; CEB; SYB3 |
| Background | Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. VAMP3 is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Because of its high homology to other known VAMPs, its broad tissue distribution, and its subcellular localization, the protein was shown to be the human equivalent of the rodent cellubrevin. In platelets the protein resides on a compartment that is not mobilized to the plasma membrane on calcium or thrombin stimulation. VAMP3 is involved in vesicular transport from the late endosomes to the trans-Golgi network. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(ICC) | 1:50-200 |
| IHC-P | 1:200-400 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is VAMP3 (8A3) Monoclonal Antibody (bsm-54366R) validated for, and at what dilution?
bsm-54366R is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(ICC) (1:50-200); IHC-P (1:200-400); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does VAMP3 (8A3) Monoclonal Antibody (bsm-54366R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-54366R be stored?
Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise VAMP3 (8A3) Monoclonal Antibody (bsm-54366R)?
Synthetic peptide conjugated to KLH within N-terminal human VAMP3/Cellubrevin.