Nicastrin (3F10) Monoclonal Antibody

$Lane 1: THP-1 Cell; Probed with Nicastrin (3F10) Monoclonal Antibody (bsm-54373R) at 1:1000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: THP-1 Cell; Probed with Nicastrin (3F10) Monoclonal Antibody (bsm-54373R) at 1:1000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

Reactivity

Human

Overview
Catalog #	bsm-54373R
Product Name	Nicastrin (3F10) Monoclonal Antibody
Applications	WB
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic peptide within Human Nicastrin aa 650-750 (C terminal).
Clonality	Monoclonal
Clone #	3F10
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	23385
Swiss Prot	Q92542
Subcellular location	Extracellular, Membrane bound, Cell membrane
Synonyms	Nicastrin; NCSTN; KIAA0253; ATAG1874
Background	Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). It probably represents a stabilizing cofactor required for the assembly of the gamma-secretase complex.
Application Dilution
WB	1:300-5000