Cullin 2 (4C9) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54381R-100uL |
| Product Name | Cullin 2 (4C9) Monoclonal Antibody |
| Applications |
WB
IF(ICC)
IHC-P
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Recombinant protein within human Cullin 2 aa 1-200. |
| Clonality | Monoclonal |
| Clone # | 4C9 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Target | |
| Gene ID | 8453 |
| Swiss Prot | Q13617 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Cullin-2; CUL-2; CUL2 |
| Background | Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. ECS complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins (PubMed:27565346). May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(ICC) | 1:50-200 |
| IHC-P | 1:200-400 |
Frequently asked questions
Which applications is Cullin 2 (4C9) Monoclonal Antibody (bsm-54381R) validated for, and at what dilution?
bsm-54381R is validated for WB (1:300-5000); IF(ICC) (1:50-200); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Cullin 2 (4C9) Monoclonal Antibody (bsm-54381R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-54381R be stored?
Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Cullin 2 (4C9) Monoclonal Antibody (bsm-54381R)?
Recombinant protein within human Cullin 2 aa 1-200.