GOPC (1B8) Monoclonal Antibody

$Lane 1: SiHa Cells; Probed with GOPC (1B8) Monoclonal Antibody (bsm-54392R) at 1:1000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: SiHa Cells; Probed with GOPC (1B8) Monoclonal Antibody (bsm-54392R) at 1:1000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with GOPC (1B8) Monoclonal Antibody (bsm-54392R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with GOPC (1B8) Monoclonal Antibody (bsm-54392R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with GOPC (1B8) Monoclonal Antibody (bsm-54392R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with GOPC (1B8) Monoclonal Antibody (bsm-54392R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with GOPC (1B8) Monoclonal Antibody (bsm-54392R) at 1:100 in SKOV-3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with GOPC (1B8) Monoclonal Antibody (bsm-54392R) at 1:100 in SKOV-3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Human Liver Cancer tissue incubated with GOPC (1B8) Monoclonal Antibody (bsm-54392R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Liver Cancer tissue incubated with GOPC (1B8) Monoclonal Antibody (bsm-54392R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Colon tissue incubated with GOPC (1B8) Monoclonal Antibody (bsm-54392R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Colon tissue incubated with GOPC (1B8) Monoclonal Antibody (bsm-54392R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with GOPC (1B8) Monoclonal Antibody (bsm-54392R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with GOPC (1B8) Monoclonal Antibody (bsm-54392R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Brian tissue incubated with GOPC (1B8) Monoclonal Antibody (bsm-54392R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Brian tissue incubated with GOPC (1B8) Monoclonal Antibody (bsm-54392R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Flow cytometric analysis of MCF-7 cells with GOPC (1B8) Monoclonal Antibody (bsm-54392R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).

Applications

WB
FCM
IHC-P
IF(IHC-P)
IF(ICC)
IP

Reactivity

Human
Mouse

Overview
Catalog #	bsm-54392R
Product Name	GOPC (1B8) Monoclonal Antibody
Applications	WB FCM IHC-P IF(IHC-P) IF(ICC) IP
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within human PIST aa 1-100.
Clonality	Monoclonal
Clone #	1B8
Isotype	IgG
Concentration	Lot Dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	57120
Swiss Prot	Q9HD26
Subcellular location	Cytoplasm, Cell membrane, Golgi apparatus
Synonyms	Golgi-associated PDZ and coiled-coil motif-containing protein; CFTR-associated ligand; Fused in glioblastoma; PDZ protein interacting specifically with TC10; PIST; GOPC
Background	Plays a role in intracellular protein trafficking and degradation. May regulate CFTR chloride currents and acid-induced ASIC3 currents by modulating cell surface expression of both channels. May also regulate the intracellular trafficking of the ADR1B receptor. May play a role in autophagy. Overexpression results in CFTR intracellular retention and degradation in the lysosomes.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(IHC-P)	1:50-200
IF(ICC)	1:50-200
IP	1-2ug