GLI3 Recombinant Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54400r-100uL |
| Product Name | GLI3 Recombinant Antibody |
| Applications |
WB
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Recombinant protein within human Gli3 aa 1300-1600. |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 2737 |
| Swiss Prot | P10071 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Transcriptional activator GLI3; GLI3 form of 190 kDa; GLI3 full-length protein; Transcriptional repressor GLI3R; GLI3 C-terminally truncated form; GLI3 form of 83 kDa; GLI3-190; GLI3FL; GLI3-83; |
| Background | Has a dual function as a transcriptional activator and a repressor of the sonic hedgehog (Shh) pathway, and plays a role in limb development. The full-length GLI3 form (GLI3FL) after phosphorylation and nuclear translocation, acts as an activator (GLI3A) while GLI3R, its C-terminally truncated form, acts as a repressor. A proper balance between the GLI3 activator and the repressor GLI3R, rather than the repressor gradient itself or the activator/repressor ratio gradient, specifies limb digit number and identity. In concert with TRPS1, plays a role in regulating the size of the zone of distal chondrocytes, in restricting the zone of PTHLH expression in distal cells and in activating chondrocyte proliferation. Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3'. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is GLI3 Recombinant Antibody (bsm-54400R) validated for, and at what dilution?
bsm-54400R is validated for WB (1:300-5000); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GLI3 Recombinant Antibody (bsm-54400R) react with?
Tested reactivity: Human.
How should bsm-54400R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
What immunogen was used to raise GLI3 Recombinant Antibody (bsm-54400R)?
Recombinant protein within human Gli3 aa 1300-1600.