Xanthine Oxidase (2D9) Monoclonal Antibody

$Lane 1: Human Kidney; Probed with Xanthine Oxidase (2D9) Monoclonal Antibody (bsm-54401R) at 1:1000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Human Kidney; Probed with Xanthine Oxidase (2D9) Monoclonal Antibody (bsm-54401R) at 1:1000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Rat Liver tissue incubated with Xanthine Oxidase (2D9) Monoclonal Antibody (bsm-54401R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat Liver tissue incubated with Xanthine Oxidase (2D9) Monoclonal Antibody (bsm-54401R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Liver tissue incubated with Xanthine Oxidase (2D9) Monoclonal Antibody (bsm-54401R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Liver tissue incubated with Xanthine Oxidase (2D9) Monoclonal Antibody (bsm-54401R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
IHC-P

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54401R
Product Name	Xanthine Oxidase (2D9) Monoclonal Antibody
Applications	WB, IHC-P
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within human Xanthine Oxidase aa 100-300.
Clonality	Monoclonal
Clone #	2D9
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	7498
Swiss Prot	P47989
Subcellular location	Cytoplasm, Secreted
Synonyms	Xanthine dehydrogenase/oxidase; Xanthine dehydrogenase; Xanthine oxidase; Xanthine oxidoreductase; XD; XO; XOR; XDH; XDHA
Background	The process of metabolizing purines to a common molecule known as xanthine is an essential process for the proper shuttling of uric acid. Xanthine oxidase is a flavoprotein enzyme that coordinates molybdenum and utilizes NAD+ as an electron acceptor to catalyze the oxidation of hypoxanthine to xanthine and then to uric acid. The predominant form of this enzyme is xanthine dehydrogenase, which is a homodimer that can be converted to xanthine oxidase by sulfhydryl oxidation or proteolytic modification. Xanthine oxidase is present in species ranging from bacteria to human and is ubiquitously expressed in mammalian tissues. In the oxidase form, this enzyme is coupled to the generation of free radicals. Individuals showing marked elevation of serum xanthine oxidase is suggestive of chronic liver disease and cholestasis, which is a condition defined by hepatic obstruction. Hepatic obstruction causes bile salts, the bile pigment bilirubin, and fats to accumulate in the blood stream instead of being eliminated normally. The clinical consequences of defects in xanthine oxidase range from mild to severe and even contribute to fatal disorders.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400

Applications

Reactivity

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