hUPF1 Recombinant Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-54402r-bf488 |
| Product Name | hUPF1 Recombinant Antibody, AbBy Fluor-488 Conjugated |
| Applications |
FCM
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | Recombinant protein within human hUPF1 aa 1-200. |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5976 |
| Swiss Prot | Q92900 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Regulator of nonsense transcripts 1; UPF1 RNA helicase and ATPase; ATP-dependent helicase RENT1; Nonsense mRNA reducing factor 1; Up-frameshift suppressor 1 homolog; NORF1; hUpf1; KIAA0221; RENT1 |
| Background | RNA-dependent helicase and ATPase required for nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Is recruited to mRNAs upon translation termination and undergoes a cycle of phosphorylation and dephosphorylation; its phosphorylation appears to be a key step in NMD. Recruited by release factors to stalled ribosomes together with the SMG1C protein kinase complex to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) (located 50-55 or more nucleotides downstream from the termination codon) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Phosphorylated UPF1 is recognized by EST1B/SMG5, SMG6 and SMG7 which are thought to provide a link to the mRNA degradation machinery involving exonucleolytic and endonucleolytic pathways, and to serve as adapters to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation and allowing the recycling of NMD factors. UPF1 can also activate NMD without UPF2 or UPF3, and in the absence of the NMD-enhancing downstream EJC indicative for alternative NMD pathways. Plays a role in replication-dependent histone mRNA degradation at the end of phase S; the function is independent of UPF2. For the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed. The ATPase activity of UPF1 is required for disassembly of mRNPs undergoing NMD. Essential for embryonic viability. |
| Application Dilution | |
| FCM | 1ug/Test |
| IF(ICC) | IFICC1:100-500 |
Frequently asked questions
Which applications is hUPF1 Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-54402R-BF488) validated for, and at what dilution?
bsm-54402R-BF488 is validated for FCM(FCM) (1ug/Test); IF(ICC)(IF(ICC)) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does hUPF1 Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-54402R-BF488) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse.
How should bsm-54402R-BF488 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise hUPF1 Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-54402R-BF488)?
Recombinant protein within human hUPF1 aa 1-200.
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bsm-54402R-BF488)?
AbBy Fluor-488 on bsm-54402R-BF488: excitation / emission 499nm/519nm.