NONO (1G11) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54412R-100uL |
| Product Name | NONO (1G11) Monoclonal Antibody |
| Applications |
WB
IF(IHC-P)
IF(ICC)
IHC-P
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Recombinant protein of C-terminal human p54nrb. |
| Clonality | Monoclonal |
| Clone # | 1G11 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 4841 |
| Swiss Prot | Q15233 |
| Subcellular location | Nucleus |
| Synonyms | Non-POU domain-containing octamer-binding protein; 54 kDa nuclear RNA- and DNA-binding protein; 55 kDa nuclear protein; DNA-binding p52/p100 complex 52 kDa subunit; NMT55; p54(nrb); p54nrb; NONO; NRB54 |
| Background | DNA- and RNA binding protein, involved in several nuclear processes. Binds the conventional octamer sequence in double stranded DNA. Also binds single-stranded DNA and RNA at a site independent of the duplex site. Involved in pre-mRNA splicing, probably as an heterodimer with SFPQ. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1. The SFPQ-NONO heteromer may be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends. In vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. Nono is involved in transcriptional regulation. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(ICC) | 1:50-200 |
| IHC-P | 1:200-400 |
Frequently asked questions
Which applications is NONO (1G11) Monoclonal Antibody (bsm-54412R) validated for, and at what dilution?
bsm-54412R is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(ICC) (1:50-200); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NONO (1G11) Monoclonal Antibody (bsm-54412R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-54412R be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NONO (1G11) Monoclonal Antibody (bsm-54412R)?
Recombinant protein of C-terminal human p54nrb.