CHAF1A (1D4) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54414R-100uL |
| Product Name | CHAF1A (1D4) Monoclonal Antibody |
| Applications |
WB
IF(IHC-P)
IF(ICC)
IP
IHC-P
FCM
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Recombinant protein within human p150 CAF1 aa 100-300. |
| Clonality | Monoclonal |
| Clone # | 1D4 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 10036 |
| Swiss Prot | Q13111 |
| Subcellular location | Nucleus |
| Synonyms | Chromatin assembly factor 1 subunit A; Chromatin assembly factor I p150 subunit; CAF-1 subunit A; CAF-I 150 kDa subunit; CAF-I p150; hp150; p150 CAF1 |
| Background | Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(ICC) | 1:50-200 |
| IP | 1-2ug |
| IHC-P | 1:200-400 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is CHAF1A (1D4) Monoclonal Antibody (bsm-54414R) validated for, and at what dilution?
bsm-54414R is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(ICC) (1:50-200); IP (1-2ug); IHC-P (1:200-400); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CHAF1A (1D4) Monoclonal Antibody (bsm-54414R) react with?
Tested reactivity: Human.
How should bsm-54414R be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CHAF1A (1D4) Monoclonal Antibody (bsm-54414R)?
Recombinant protein within human p150 CAF1 aa 100-300.