HYOU1 (5H4) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54415R-100uL |
| Product Name | HYOU1 (5H4) Monoclonal Antibody |
| Applications |
WB
IHC-P
FCM
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Recombinant protein within human ORP150 aa 800-950. |
| Clonality | Monoclonal |
| Clone # | 5H4 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 10525 |
| Swiss Prot | Q9Y4L1 |
| Subcellular location | Endoplasmic reticulum |
| Synonyms | Hypoxia up-regulated protein 1; 150 kDa oxygen-regulated protein; 170 kDa glucose-regulated protein; ORP-150; GRP-170; HYOU1; GRP170; ORP150 |
| Background | ORP150, also known as HYOU1 (hypoxia upregulated 1), is a 999 amino acid protein that localizes to the lumen of the endoplasmic reticulum (ER) and is a member of the heat shock protein 70 (HSP 70) family. ORP150 is highly expressed in tissues that have well-developed ERs and a large number of secretory proteins (such as liver and pancreas). It is expressed at lower levels in kidney and brain, and plays an essential role in cytoprotective cellular responses to hypoxia (oxygen deprivation). Specifically, ORP150 participates in protein folding and secretion in the ER and functions to protect cells from hypoxia-induced apoptosis, thereby playing a crucial role in cell survival. ORP150 expression is upregulated in a variety of tumors, such as breast cancer, suggesting an important role in tumorigenesis. The gene encoding ORP150 has two translation initiation sites, resulting in a truncated transcript that lacks an ER signal peptide, but is thought to function as a housekeeping protein in the cytoplasm. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-P | 1:200-400 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is HYOU1 (5H4) Monoclonal Antibody (bsm-54415R) validated for, and at what dilution?
bsm-54415R is validated for WB (1:300-5000); IHC-P (1:200-400); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HYOU1 (5H4) Monoclonal Antibody (bsm-54415R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-54415R be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HYOU1 (5H4) Monoclonal Antibody (bsm-54415R)?
Recombinant protein within human ORP150 aa 800-950.