MARK2 (1F10) Monoclonal Antibody

$Lane 1: MCF-7 Cells; Lane 2: SK-Br-3 Cells; Probed with MARK2 (1F10) Monoclonal Antibody (bsm-54447R) at 1:2000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: MCF-7 Cells; Lane 2: SK-Br-3 Cells; Probed with MARK2 (1F10) Monoclonal Antibody (bsm-54447R) at 1:2000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Human Appendix tissue incubated with MARK2 (1F10) Monoclonal Antibody (bsm-54447R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Appendix tissue incubated with MARK2 (1F10) Monoclonal Antibody (bsm-54447R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Flow cytometric analysis of MCF-7 cells with MARK2 (1F10) Monoclonal Antibody (bsm-54447R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).

Applications

WB
FCM
IHC-P

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54447R
Product Name	MARK2 (1F10) Monoclonal Antibody
Applications	WB, FCM, IHC-P
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein near the C-terminus of human MARK2.
Clonality	Monoclonal
Clone #	1F10
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	2011
Swiss Prot	Q7KZI7
Subcellular location	Cytoplasm, Cell membrane, Cytoskeleton
Synonyms	Serine/threonine-protein kinase MARK2; ELKL motif kinase 1; MAP/microtubule affinity-regulating kinase 2; EMK-1; Par-1b; Par1b; MARK2; EMK1
Background	MARK2 refers to MAP/microtubule affinity-regulating kinase 2 isoform a [Homo sapiens]. EMK (ELKL Motif Kinase) is a small family of ser/thr protein kinases involved in the control of cell polarity, microtubule.stability and cancer. Several cDNA clones have been isolated that encoded two isoforms of the human ser/thr protein kinase EMK1 called MARK2. These isoforms were characterized by the presence of a 162-bp alternative exon that gave rise to two forms, one containing the exon and the other one lacking it. Both forms were found to be coexpressed in a number of selected cell lines and tissue samples. Human MARK2 was shown to be encoded by a single mRNA ubiquitously expressed. This transcription variant includes the alternative exon in the coding region and therefore codes for a longer protein. Multiple splice variants exist for this protein.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400

Applications

Reactivity

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