Fbx32 Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with Fbx32 monoclonal antibody, unconjugated (bsm-54451R) at 1:10000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with Fbx32 monoclonal antibody, unconjugated (bsm-54451R) at 1:10000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
ICC/IF

Reactivity

Mouse
Rat

Predicted Reactivity

Human

Overview
Catalog #	bsm-54451r-TR
Product Name	Fbx32 Recombinant Antibody
Applications	WB, ICC/IF
Reactivity	Mouse, Rat
Predicted Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant human Fbx32
Immunogen Range	1-200/355
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	114907
Swiss Prot	Q969P5
Subcellular location	Cytoplasm, Nucleus
Synonyms	Fbx32; 4833442G10Rik; AI430017; Atrogin 1; Atrogin-1; ATROGIN1; Atrophy gene 1; F box only protein 32; F-box only protein 32; F-box protein 32; FBX32_HUMAN; fbxo25; FBXO32; FLJ32424; MAFbx; MGC108443; MGC137646; MGC33610; Muscle atrophy F box; Muscle atrophy F box protein; Muscle atrophy F-box protein.
Background	Substrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1.
Application Dilution
WB	1:1000-2000
ICC/IF	1:50-200