TBXT Recombinant Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54459R-100uL |
| Product Name | TBXT Recombinant Antibody |
| Applications |
WB
IF(ICC)
IP
IHC-P
FCM
|
| Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Recombinant protein within human TBXT aa 200-400. |
| Clonality | Monoclonal |
| Clone # | 1F11 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 6862 |
| Swiss Prot | O15178 |
| Subcellular location | Nucleus |
| Synonyms | T-box transcription factor T; TBXT; Brachyury protein |
| Background | TBXTis an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. Variation in this gene was associated with susceptibility to neural tube defects and chordoma. A mutation in this gene was found in a family with sacral agenesis with vertebral anomalies. Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(ICC) | 1:50-200 |
| IP | 1-2ug |
| IHC-P | 1:200-400 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is TBXT Recombinant Antibody (bsm-54459R) validated for, and at what dilution?
bsm-54459R is validated for WB (1:300-5000); IF(ICC) (1:50-200); IP (1-2ug); IHC-P (1:200-400); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does TBXT Recombinant Antibody (bsm-54459R) react with?
Tested reactivity: Human, Mouse.
How should bsm-54459R be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TBXT Recombinant Antibody (bsm-54459R)?
Recombinant protein within human TBXT aa 200-400.