MARK3 Recombinant Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-54462r-cy3-100ul |
| Product Name | MARK3 Recombinant Antibody, Cy3 Conjugated |
| Applications |
WB
|
| Reactivity | Human |
| Predicted Reactivity | Mouse |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | Recombinant protein within human Mark3 aa 600-750. |
| Clonality | Monoclonal |
| Clone # | 2F7 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4140 |
| Swiss Prot | P27448 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | MAP/microtubule affinity-regulating kinase 3; C-TAK1; cTAK1; Cdc25C-associated protein kinase 1; ELKL motif kinase 2; EMK-2; Protein kinase STK10; Ser/Thr protein kinase PAR-1; Par-1a; Serine/threonine-protein kinase p78; microtubule affinity regulating kinase 3 |
| Background | Serine/threonine-protein kinase. Involved in the specific phosphorylation of microtubule-associated proteins for MAP2 and MAP4. Phosphorylates the microtubule-associated protein MAPT/TAU. Phosphorylates CDC25C on 'Ser-216'. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus. Negatively regulates the Hippo signaling pathway and antagonizes the phosphorylation of LATS1. Cooperates with DLG5 to inhibit the kinase activity of STK3/MST2 toward LATS1. |
| Application Dilution | |
| WB | 1:500-2000 |
Frequently asked questions
Which applications is MARK3 Recombinant Antibody, Cy3 Conjugated (bsm-54462R-Cy3) validated for, and at what dilution?
bsm-54462R-Cy3 is validated for WB (1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does MARK3 Recombinant Antibody, Cy3 Conjugated (bsm-54462R-Cy3) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse.
How should bsm-54462R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MARK3 Recombinant Antibody, Cy3 Conjugated (bsm-54462R-Cy3)?
Recombinant protein within human Mark3 aa 600-750.
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-54462R-Cy3)?
Cy3 on bsm-54462R-Cy3: excitation / emission 512,550nm/570,615nm.