Phospho-Erk1(T202)+Erk2(T185) Monoclonal Antibody, APC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54491r-apc-100uL |
| Product Name | Phospho-Erk1(T202)+Erk2(T185) Monoclonal Antibody, APC Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | Synthetic phospho-Peptide corresponding to residues surrounding Thr185 of human Erk2. |
| Modification Site | Thr202+Thr185 |
| Clonality | Monoclonal |
| Clone # | 6H2 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5595 |
| Swiss Prot | P27361 |
| Subcellular location | Cytoplasm, Nucleus |
| Background | The protein encoded by this gene is a member of the MAPkinase family. MAP kinases, also known as extracellularsignal-regulated kinases (ERKs), act in a signaling cascade thatregulates various cellular processes such as proliferation,differentiation, and cell cycle progression in response to avariety of extracellular signals. This kinase is activated byupstream kinases, resulting in its translocation to the nucleuswhere it phosphorylates nuclear targets. Alternatively splicedtranscript variants encoding different protein isoforms have beendescribed. [provided by RefSeq, Jul 2008]. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:50-200 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is Phospho-Erk1(T202)+Erk2(T185) Monoclonal Antibody, APC Conjugated (bsm-54491R-APC) validated for, and at what dilution?
bsm-54491R-APC is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:50-200); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-Erk1(T202)+Erk2(T185) Monoclonal Antibody, APC Conjugated (bsm-54491R-APC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-54491R-APC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-Erk1(T202)+Erk2(T185) Monoclonal Antibody, APC Conjugated (bsm-54491R-APC)?
Synthetic phospho-Peptide corresponding to residues surrounding Thr185 of human Erk2.
What are the excitation and emission wavelengths of the APC conjugate (bsm-54491R-APC)?
APC on bsm-54491R-APC: excitation / emission 650nm/660nm.