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Cytokeratin 8 Monoclonal Antibody

Applications

  • WB
  • IF(ICC)
  • IP
  • IHC
  • IHC-P
  • FCM

Reactivity

  • Human
  • Mouse
Overview
Catalog # bsm-54512R-100uL
Product Name Cytokeratin 8 Monoclonal Antibody
Applications
WB IF(ICC) IP IHC IHC-P FCM
Reactivity Human, Mouse
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant protein
Clonality Monoclonal
Clone # 5F3
Isotype IgG
Concentration Lot Dependent
Purification purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 3875
Swiss Prot P05783
Subcellular location Cytoplasm, Nucleus, Nucleus matrix
Synonyms CARD2 antibody, Cell proliferation inducing gene 46 protein antibody, Cell proliferation inducing protein 46 antibody, CK 8 antibody, CK-8 antibody, CK8 antibody, CYK8 antibody, Cytokeratin 8 antibody, Cytokeratin-8 antibody, K2C8 antibody, K2C8_HUMAN antibody, K8 antibody, Keratin 8 antibody, Keratin antibody, keratin type II cytoskeletal 8 antibody, Keratin-8 antibody, KRT8 antibody, type II cytoskeletal 8 antibody, Type-II keratin Kb8 antibody
Background KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Application Dilution
WB 1:300-5000
IF(ICC) 1:50-200
IP 1-2ug
IHC 1:50-200
IHC-P 1:200-400
FCM 1:20-100

Frequently asked questions

Which applications is Cytokeratin 8 Monoclonal Antibody (bsm-54512R) validated for, and at what dilution?

bsm-54512R is validated for WB (1:300-5000); IF(ICC) (1:50-200); IP (1-2ug); IHC (1:50-200); IHC-P (1:200-400); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Cytokeratin 8 Monoclonal Antibody (bsm-54512R) react with?

Tested reactivity: Human, Mouse.

How should bsm-54512R be stored?

Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Cytokeratin 8 Monoclonal Antibody (bsm-54512R)?

Recombinant protein

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US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

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