Phospho-PAK1(S144)+PAK2(S141)+PAK3(S139) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54514R-100uL |
| Product Name | Phospho-PAK1(S144)+PAK2(S141)+PAK3(S139) Monoclonal Antibody |
| Applications |
WB
IF(ICC)
IP
IHC
IHC-P
FCM
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Synthetic phospho-Peptide corresponding to residues surrounding Ser144 of human PAK1. |
| Modification Site | Ser144+Ser139+Ser141 |
| Clonality | Monoclonal |
| Clone # | 7G3 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 5063 |
| Swiss Prot | O75914 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | , |
| Background | PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, serve as targets for the small GTP binding proteins Cdc42 and RAC and have been implicated in a wide range of biological activities. The protein encoded by this gene forms an activated complex with GTP-bound RAS-like (P21), CDC2 and RAC1 proteins which then catalyzes a variety of targets. This protein may be necessary for dendritic development and for the rapid cytoskeletal reorganization in dendritic spines associated with synaptic plasticity. Defects in this gene are the cause of non-syndromic mental retardation X-linked type 30 (MRX30), also called X-linked mental retardation type 47 (MRX47). Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(ICC) | 1:50-200 |
| IP | 1-2ug |
| IHC | 1:50-200 |
| IHC-P | 1:200-400 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is Phospho-PAK1(S144)+PAK2(S141)+PAK3(S139) Monoclonal Antibody (bsm-54514R) validated for, and at what dilution?
bsm-54514R is validated for WB (1:300-5000); IF(ICC) (1:50-200); IP (1-2ug); IHC (1:50-200); IHC-P (1:200-400); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-PAK1(S144)+PAK2(S141)+PAK3(S139) Monoclonal Antibody (bsm-54514R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-54514R be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-PAK1(S144)+PAK2(S141)+PAK3(S139) Monoclonal Antibody (bsm-54514R)?
Synthetic phospho-Peptide corresponding to residues surrounding Ser144 of human PAK1.