Phospho-MEK1(S218/S222) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54527R-100uL |
| Product Name | Phospho-MEK1(S218/S222) Monoclonal Antibody |
| Applications |
WB
IF(ICC)
IP
IHC
IHC-P
FCM
|
| Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Synthetic phospho-Peptide corresponding to residues surrounding Ser218 and 222 of human MEK1. |
| Modification Site | Ser218/Ser222 |
| Clonality | Monoclonal |
| Clone # | 3B1 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 5604 |
| Swiss Prot | Q02750 |
| Subcellular location | Cytoplasm, Nucleus, Membrane |
| Synonyms | Dual specificity mitogen activated protein kinase kinase 1 antibody, Dual specificity mitogen-activated protein kinase kinase 1 antibody, ERK activator kinase 1 antibody, MAP kinase kinase 1 antibody, MAP kinase/Erk kinase 1 antibody, MAP2K1 antibody, MAPK/ERK kinase 1 antibody, MAPKK 1 antibody, MAPKK1 antibody, MEK 1 antibody, Mek1 antibody, MEKK1 antibody, Mitogen activated protein kinase kinase 1 antibody, MKK 1 antibody, MKK1 antibody, MP2K1_HUMAN antibody, PRKMK1 antibody, Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1) antibody, Protein kinase mitogen activated, kinase 1 antibody, hide |
| Background | The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(ICC) | 1:50-200 |
| IP | 1-2ug |
| IHC | 1:50-200 |
| IHC-P | 1:200-400 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is Phospho-MEK1(S218/S222) Monoclonal Antibody (bsm-54527R) validated for, and at what dilution?
bsm-54527R is validated for WB (1:300-5000); IF(ICC) (1:50-200); IP (1-2ug); IHC (1:50-200); IHC-P (1:200-400); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-MEK1(S218/S222) Monoclonal Antibody (bsm-54527R) react with?
Tested reactivity: Human, Mouse.
How should bsm-54527R be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-MEK1(S218/S222) Monoclonal Antibody (bsm-54527R)?
Synthetic phospho-Peptide corresponding to residues surrounding Ser218 and 222 of human MEK1.