Phospho-PKA R2 (S99) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54545R-100uL |
| Product Name | Phospho-PKA R2 (S99) Monoclonal Antibody |
| Applications |
WB
IF(ICC)
IP
IHC
IHC-P
|
| Reactivity | Human, Mouse, Rat, Pig |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Recombinant protein |
| Modification Site | Ser99 |
| Clonality | Monoclonal |
| Clone # | 4H11 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 5576 |
| Swiss Prot | P13861 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | cAMP dependent protein kinase regulatory subunit alpha 2 antibody, cAMP dependent protein kinase regulatory subunit RII alpha antibody, cAMP dependent protein kinase type II alpha regulatory chain antibody, cAMP dependent protein kinase type II alpha regulatory subunit antibody, cAMP-dependent protein kinase type II-alpha regulatory subunit antibody, KAP2 antibody, KAP2_HUMAN antibody, MGC3606 antibody, PKR 2 antibody, PKR2 antibody, PRKA R2 antibody, PRKAR 2 antibody, PRKAR2 antibody, PRKAR2A antibody, Protein kinase A RII alpha subunit antibody, Protein kinase cAMP dependent regulatory type II alpha antibody |
| Background | The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme, resulting in release of active catalytic subunits. Activation of transcription upon elevation of cAMP levels results from translocation of PKA to the nucleus, where it phosphorylates the transcription factor cAMP response element binding protein (CREB) on Serine 133, which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking phospho-CREB to the Pol II transcription initiation complex. Mouse Serine 96 (designated Ser 99 in human) is a phosphorylation site on the PKA II?regulatory subunit |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(ICC) | 1:50-200 |
| IP | 1-2ug |
| IHC | 1:50-200 |
| IHC-P | 1:200-400 |
Frequently asked questions
Which applications is Phospho-PKA R2 (S99) Monoclonal Antibody (bsm-54545R) validated for, and at what dilution?
bsm-54545R is validated for WB (1:300-5000); IF(ICC) (1:50-200); IP (1-2ug); IHC (1:50-200); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-PKA R2 (S99) Monoclonal Antibody (bsm-54545R) react with?
Tested reactivity: Human, Mouse, Rat, Pig.
How should bsm-54545R be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-PKA R2 (S99) Monoclonal Antibody (bsm-54545R)?
Recombinant protein