Phospho-Chk1 (S296) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54575R-100uL |
| Product Name | Phospho-Chk1 (S296) Monoclonal Antibody |
| Applications |
WB
IHC
IHC-P
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Synthetic phospho-Peptide corresponding to residues surrounding Ser296 of human Chk1 |
| Modification Site | Ser296 |
| Clonality | Monoclonal |
| Clone # | 4B6 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 1111 |
| Swiss Prot | O14757 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | C85740 antibody, Cell cycle checkpoint kinase antibody, Checkpoint , S. pombe, homolog of, 1 antibody, Checkpoint kinase 1 antibody, Checkpoint kinase 1 homolog (S. pombe) antibody, CHEK 1 antibody, Chek1 antibody, Chk 1 antibody, Chk1 antibody, CHK1 checkpoint homolog (S. pombe) antibody, CHK1_HUMAN antibody, EC 2.7.11.1 antibody, rad27 antibody, Serine/threonine protein kinase Chk1 antibody, Serine/threonine-protein kinase CHK1 antibody, STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) antibody |
| Background | The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Oct 2011]. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC | 1:50-200 |
| IHC-P | 1:200-400 |
Frequently asked questions
Which applications is Phospho-Chk1 (S296) Monoclonal Antibody (bsm-54575R) validated for, and at what dilution?
bsm-54575R is validated for WB (1:300-5000); IHC (1:50-200); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-Chk1 (S296) Monoclonal Antibody (bsm-54575R) react with?
Tested reactivity: Human.
How should bsm-54575R be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-Chk1 (S296) Monoclonal Antibody (bsm-54575R)?
Synthetic phospho-Peptide corresponding to residues surrounding Ser296 of human Chk1