Cyclin E2 Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54580R-100uL |
| Product Name | Cyclin E2 Monoclonal Antibody |
| Applications |
WB
IF(ICC)
IHC
IHC-P
FCM
|
| Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Recombinant protein |
| Clonality | Monoclonal |
| Clone # | 4F3 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 9134 |
| Swiss Prot | O96020 |
| Subcellular location | Nucleus |
| Synonyms | CCN E2 antibody, CCNE 2 antibody, CCNE2 antibody, CCNE2 protein antibody, CYC E2 antibody, CYCE 2 antibody, CYCE2 antibody, CyclinE2 antibody, G1/S specific cyclin E2 antibody |
| Background | The human Cyclin E2 gene encodes a 404 amino acid protein that is most closely related to Cyclin E. Cyclin E2 mRNA levels peaks at the G1 / S transition. Cyclin E2 associates with Cdk2 in a functional kinase complex that is inhibited by both p27 (Kip1) and p21 (Cip1). Cyclin E2 / Cdk2 phosphorylates histone H1 in vitro. G1 cyclin E controls the initiation of DNA synthesis by activating CDK2. Abnormally high levels of cyclin E expression have frequently been observed in human cancers. Unlike Cyclin E1, which is expressed in great majority of proliferating normal and neoplastically transformed cells, Cyclin E2 levels are low to undetectable in non transformed cells and increase significantly in neoplasm derived cells. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(ICC) | 1:50-200 |
| IHC | 1:50-200 |
| IHC-P | 1:200-400 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is Cyclin E2 Monoclonal Antibody (bsm-54580R) validated for, and at what dilution?
bsm-54580R is validated for WB (1:300-5000); IF(ICC) (1:50-200); IHC (1:50-200); IHC-P (1:200-400); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Cyclin E2 Monoclonal Antibody (bsm-54580R) react with?
Tested reactivity: Human, Mouse.
How should bsm-54580R be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Cyclin E2 Monoclonal Antibody (bsm-54580R)?
Recombinant protein