Phospho-PLK1(T210) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54621R |
| Product Name | Phospho-PLK1(T210) Monoclonal Antibody |
| Applications |
WB
FCM
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Synthetic phospho-Peptide corresponding to residues surrounding Thr210 of human PLK1. |
| Modification Site | Thr210 |
| Clonality | Monoclonal |
| Clone # | 1H4 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 5347 |
| Swiss Prot | P53350 |
| Subcellular location | Cytoplasm, Nucleus, Chromosome, Midbody |
| Synonyms | Cell cycle regulated protein kinase antibody, PLK 1 antibody, PLK antibody, PLK-1 antibody, plk1 antibody, PLK1_HUMAN antibody, Polo like kinase 1 antibody, Polo-like kinase 1 antibody, Serine/threonine protein kinase 13 antibody, Serine/threonine protein kinase PLK1 antibody, Serine/threonine-protein kinase 13 antibody, Serine/threonine-protein kinase PLK1 antibody, STPK 13 antibody, STPK13 antibody |
| Background | PLK1 (polo-like kinase 1) is a member of the serine/threonine protein kinase family, cdc5/polo subfamily. PLK1 contains two polo box domains with a predicted molecular weight of 68 kDa. PLK1 has been shown to regulate cdc2/cyclin B through phosphorylation and activation of cdc25c phosphatase. PLK1 is modified by phosphorylation at Threonine 210. PLK1 may also be required for cell division. Depletion of PLK1 results in apoptosis and deregulation of expression of PKL1 is correlated with development of many malignancies. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-PLK1(T210) Monoclonal Antibody (bsm-54621R) validated for, and at what dilution?
bsm-54621R is validated for WB (1:300-5000); FCM (1:20-100); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-PLK1(T210) Monoclonal Antibody (bsm-54621R) react with?
Tested reactivity: Human.
How should bsm-54621R be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-PLK1(T210) Monoclonal Antibody (bsm-54621R)?
Synthetic phospho-Peptide corresponding to residues surrounding Thr210 of human PLK1.