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Phospho-SHIP(Y1020) Monoclonal Antibody

A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37\u00b0C for 20 min; Antibody incubation with (Phospho-SHIP(Y1020)) Monoclonal Antibody, Unconjugated (bsm-54629R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (Phospho-SHIP(Y1020)) Monoclonal Antibody, Unconjugated (bsm-54629R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Applications

  • WB
  • IF(ICC)
  • IP

Reactivity

  • Human
Overview
Catalog # bsm-54629R
Product Name Phospho-SHIP(Y1020) Monoclonal Antibody
Applications WB, IF(ICC), IP
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source Synthetic phospho-Peptide corresponding to residues surrounding Tyr1020 of human SHIP.
Modification Site Tyr1020
Clonality Monoclonal
Clone # 1H11
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 3635
Swiss Prot Q92835
Subcellular location Cytoplasm. Membrane; Peripheral membrane protein. Note=Translocates to the plasma membrane when activated, translocation is probably due to different mechanisms depending on the stimulus and cell type. Partly translocated via its SH2 domain which mediates interaction with tyrosine phosphorylated receptors such as the FC-gamma-RIIB receptor (FCGR2B) or CD16/FCGR3. Tyrosine phosphorylation may also participate in membrane localization (By similarity).
Synonyms Inositol polyphosphate 5 phosphatase of 145kDa antibody, 4 antibody, 5-trisphosphate 5-phosphatase 1 antibody, hp51CN antibody, Inositol polyphosphate 5 phosphatase 145kDa antibody, Inositol polyphosphate 5 phosphatase antibody, Inositol polyphosphate-5-phosphatase of 145 kDa antibody, INPP5D antibody, MGC104855 antibody, MGC142140 antibody, MGC142142 antibody, p150Ship antibody, Phosphatidylinositol 3,4,5 trisphosphate 5 phosphatase 1 antibody, Phosphatidylinositol-3 antibody, SH2 containing inositol phosphatase isoform b antibody, SH2 domain containing inositol 5' phosphatase 1 antibody, SH2 domain containing inositol phosphatase 1 antibody, SH2 domain-containing inositol phosphatase 1 antibody, SH2 domain-containing inositol-5'-phosphatase 1 antibody, SHIP-1 antibody, SHIP1 antibody, SHIP1_HUMAN antibody, Signaling inositol polyphosphate 5 phosphatase SIP 145 antibody, SIP-145 antibody, SIP145 antibody
Background SHIP1 is a member of the inositol polyphosphate-5-phosphatase (INPP5) family and contains an N-terminal SH2 domain, an inositol phosphatase domain, and two C-terminal protein interaction domains. Expression of this protein is restricted to hematopoietic cells where its movement from the cytosol to the plasma membrane is mediated by tyrosine phosphorylation in response to multiple cytokine and B and T cell receptor activation. At the plasma membrane, the protein hydrolyzes the 5' phosphate from phosphatidylinositol (3,4,5)-trisphosphate and inositol-1,3,4,5-tetrakisphosphate, thereby affecting multiple signaling pathways. Overall the protein functions as a negative regulator of myeliod cell proliferation and survival.
Application Dilution
WB 1:300-5000
IF(ICC) 1:50-200
IP 1-2ug

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