ENPEP Recombinant Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54646R-100uL |
| Product Name | ENPEP Recombinant Antibody |
| Applications |
WB
IHC
IHC-P
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Synthetic Peptide within Human AP-A. |
| Clonality | Monoclonal |
| Clone # | 11B3 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 2028 |
| Swiss Prot | Q07075 |
| Subcellular location | Cell membrane, Membrane |
| Synonyms | Aminopeptidase A antibody, AMPE_HUMAN antibody, AP-A antibody, APA antibody, bp-1 antibody, bp1 antibody, CD249 antibody, Differentiation antigen gp160 antibody, EAP antibody, Enpep antibody, Glutamyl aminopeptidase (aminopeptidase A) antibody, Glutamyl aminopeptidase antibody, Gp160 antibody, Ly51 antibody |
| Background | Aminopeptidase A, also designated APA, gp160 human kidney differentiation antigen, glutamyl aminopeptidase, or enpep, is a differentiation-related kidney glycoprotein. As a cell surface, zinc-dependent metalloprotease, Aminopepti-dase A specifically cleaves amino-terminal acidic residues from peptide substrates such as Angiotensin II. APA is expressed on the surface of epithelial cells of the glomerulus and proximal tubule cells of the human nephron, where it may mediate the constitutive trafficking of Glut4-containing vesicles. These Glut4-containing vesicles are tissue-specific secretory-like microsomal structures that mediate insulin-dependent translocation of GLUT4 to the cell surface in fat and muscle cells. Mutations in the gp160/APA gene, including loss of protein expression or enzymatic activity, occur in 20% of primary clear cell renal carcinomas. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC | 1:50-200 |
| IHC-P | 1:200-400 |
Frequently asked questions
Which applications is ENPEP Recombinant Antibody (bsm-54646R) validated for, and at what dilution?
bsm-54646R is validated for WB (1:300-5000); IHC (1:50-200); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ENPEP Recombinant Antibody (bsm-54646R) react with?
Tested reactivity: Human.
How should bsm-54646R be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ENPEP Recombinant Antibody (bsm-54646R)?
Synthetic Peptide within Human AP-A.