Tryptophanyl tRNA synthetase / WRS Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Tryptophanyl tRNA synthetase \/ WRS Monoclonal Antibody, Unconjugated (bsm-54661R) at 1:200 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Tryptophanyl tRNA synthetase \/ WRS Monoclonal Antibody, Unconjugated (bsm-54661R) at 1:200 for 30 minutes at room temperature, DAB staining.

$Flow cytometric analysis of Tryptophanyl tRNA synthetase \/ WRS was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (bsm-54661R, 1\/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1\/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).$

Flow cytometric analysis of Tryptophanyl tRNA synthetase \/ WRS was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (bsm-54661R, 1\/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1\/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Applications

WB
FCM
IHC-P
IHC

Reactivity

Human

Overview
Catalog #	bsm-54661R
Product Name	Tryptophanyl tRNA synthetase / WRS Monoclonal Antibody
Applications	WB, FCM, IHC-P, IHC
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic Peptide within C-terminal human Tryptophanyl tRNA synthetase/WRS.
Clonality	Monoclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Swiss Prot	P23381
Subcellular location	Cytoplasm
Synonyms	Gamma 2 antibody, GAMMA-2 antibody, Gamma2 antibody, hWRS antibody, IFI 53 antibody, IFI53 antibody, IFP 53 antibody, IFP53 antibody, Interferon induced protein 53 antibody, Interferon-induced protein 53 antibody, SYWC_HUMAN antibody, T2-TrpRS antibody, TrpRS antibody, Tryptophan tRNA ligase, cytoplasmic antibody, Tryptophan tRNA ligase 1 cytoplasmic antibody, Tryptophan tRNA ligase antibody, Tryptophan--tRNA ligase antibody, Tryptophanyl tRNA synthetase antibody, Tryptophanyl tRNA synthetase cytoplasmic antibody, WARS antibody, WARS protein antibody, WRS antibody
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IHC