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GCDFP15 Recombinant Antibody

25 ug total protein per lane of various lysates (see on figure) probed with GCDFP15 monoclonal antibody, unconjugated (bsm-54667R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with GCDFP15 monoclonal antibody, unconjugated (bsm-54667R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GCDFP15 Monoclonal Antibody, Unconjugated (bsm-54667R) at 1:300 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GCDFP15 Monoclonal Antibody, Unconjugated (bsm-54667R) at 1:300 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GCDFP15 Monoclonal Antibody, Unconjugated (bsm-54667R) at 1:300 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GCDFP15 Monoclonal Antibody, Unconjugated (bsm-54667R) at 1:300 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF(IHC-P)

Reactivity

  • Human
Overview
Catalog # bsm-54667r
Product Name GCDFP15 Recombinant Antibody
Applications
WB IHC-P IHC-F IF(IHC-P)
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source Synthetic Peptide within N-terminal human GCDFP 15.
Clonality Recombinant
Isotype IgG
Concentration Lot Dependent
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 5304
Swiss Prot P12273
Subcellular location Secreted
Synonyms BRST-2 antibody, BRST2 antibody, GCDFP-15 antibody, GCDGP15 antibody, gp17 antibody, GPIP4 antibody, Gross cystic disease fluid protein 15 antibody, PIP antibody, PIP_HUMAN antibody, Prolactin induced protein antibody, Prolactin inducible protein precursor antibody, Prolactin-induced protein antibody, Prolactin-inducible protein antibody, SABP antibody, Secretory actin binding protein antibody, Secretory actin-binding protein antibody
Background Gross cystic disease is a common premenopausal disorder in which gross cysts are the predominant pathologic lesion. It is characterized by production of a fluid secretion which accumulates in the breast cysts. Gross cystic disease fluid is a pathologic secretion from breast composed of several glycoproteins, including a unique 15 kDa monomer protein, GCDFP 15. The cells within the body that produce GCDFP 15 appear to be restricted primarily to those with apocrine function. Studies have found GCDFP 15 to be a highly specific and sensitive marker for breast cancer.
Application Dilution
WB 1:500-2000
IHC-P IHC-P1:100-500
IHC-F IHC-F1:100-500
IF(IHC-P) IFIHC-P1:100-500

Frequently asked questions

Which applications is GCDFP15 Recombinant Antibody (bsm-54667R) validated for, and at what dilution?

bsm-54667R is validated for WB(WB) (1:500-2000); IHC-P(IHC-P) (1:100-500); IHC-F(IHC-F) (1:100-500); IF(IHC-P)(IF(IHC-P)) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does GCDFP15 Recombinant Antibody (bsm-54667R) react with?

Tested reactivity: Human.

How should bsm-54667R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

What immunogen was used to raise GCDFP15 Recombinant Antibody (bsm-54667R)?

Synthetic Peptide within N-terminal human GCDFP 15.

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