WNT5B Monoclonal Antibody

$Flow cytometric analysis of WNT5B was done on LOVO cells. The cells were fixed, permeabilized and stained with the primary antibody (bsm-54708R, 1\/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1\/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).$

Flow cytometric analysis of WNT5B was done on LOVO cells. The cells were fixed, permeabilized and stained with the primary antibody (bsm-54708R, 1\/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1\/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Applications

Reactivity

Human

Overview
Catalog #	bsm-54708R
Product Name	WNT5B Monoclonal Antibody
Applications	WB, FCM
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic Peptide within C terminal human Wnt5b.
Clonality	Monoclonal
Clone #	6H7
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	81029
Swiss Prot	Q9H1J7
Subcellular location	Extracellular matrix
Synonyms	MGC2648 antibody, Protein Wnt-5b antibody, Wingless type MMTV integration site family, member 5B antibody, WNT 5B antibody, WNT 5B protein antibody, Wnt family member 5B antibody, Wnt5b antibody, WNT5B protein antibody, WNT5B_HUMAN antibody
Background	The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 94% and 80% amino acid identity to the mouse Wnt5b protein and the human WNT5A protein, respectively. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq, Jul 2008]
Application Dilution
WB	1:300-5000
FCM	1:20-100

Applications

Reactivity

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