TGN46 Recombinant Antibody

$Lane 1: Human MCF-7 cell lysates; Lane 2: Human MDA-MB-231 cell lysates; Lane 3: Human HeLa cell lysates; Lane 4: Human HepG2 cell lysates; Lane 5: Human U251 cell lysates; Lane 6: Human A549 cell lysates probed with TGN46 Monoclonal Antibody, Unconjugated (bsm-54769R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: Human MCF-7 cell lysates; Lane 2: Human MDA-MB-231 cell lysates; Lane 3: Human HeLa cell lysates; Lane 4: Human HepG2 cell lysates; Lane 5: Human U251 cell lysates; Lane 6: Human A549 cell lysates probed with TGN46 Monoclonal Antibody, Unconjugated (bsm-54769R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

$Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TGN46 Monoclonal Antibody, Unconjugated (bsm-54769R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TGN46 Monoclonal Antibody, Unconjugated (bsm-54769R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TGN46 Monoclonal Antibody, Unconjugated (bsm-54769R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TGN46 Monoclonal Antibody, Unconjugated (bsm-54769R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TGN46 Monoclonal Antibody, Unconjugated (bsm-54769R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TGN46 Monoclonal Antibody, Unconjugated (bsm-54769R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TGN46 Monoclonal Antibody, Unconjugated (bsm-54769R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TGN46 Monoclonal Antibody, Unconjugated (bsm-54769R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TGN46 Monoclonal Antibody, Unconjugated (bsm-54769R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TGN46 Monoclonal Antibody, Unconjugated (bsm-54769R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Applications

Reactivity

Human

Predicted Reactivity

Mouse

Overview
Catalog #	bsm-54769r
Product Name	TGN46 Recombinant Antibody
Applications	WB
Reactivity	Human
Predicted Reactivity	Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human TGN46
Clonality	Recombinant
Isotype	IgG
Concentration	Lot Dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	10618
Swiss Prot	O43493
Subcellular location	Cell membrane
Synonyms	TGN 46; TGN 51; TGN38; TGN38 homolog; TGN38B; TGN46; TGN48; TGN51; TGOLN2; TGON2_HUMAN; Trans Golgi network integral membrane protein 2; Trans golgi network protein 2; Trans Golgi network protein TGN51; Trans-Golgi network integral membrane protein 1; Trans-Golgi network integral membrane protein 2; Trans-Golgi network protein TGN51; TTGN2.
Application Dilution
WB	1:500-2000