Caspase-3 Monoclonal Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-55032m-rbitc-100uL |
| Product Name | Caspase-3 Monoclonal Antibody, RBITC Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | RBITC |
| Host | Mouse |
| Source | Recombinant human Caspase-3 protein |
| Clonality | Monoclonal |
| Clone # | 1B1 |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 836 |
| Swiss Prot | P42574 |
| Subcellular location | Cytoplasm |
| Synonyms | Caspase-3 subunit p17; cleaved Caspase 3; cleaved Caspase-3; APOPAIN; CASP3; Caspase 3 apoptosis related cysteine protease; Caspase3; CPP32; CPP32B; Cysteine protease CPP32; Human cysteine protease CPP32 isoform alpha mRNA complete cds; PARP cleavage protease; SCA 1; SCA1; SREBP cleavage activity 1; Yama; CASP3_HUMAN; Caspase-3; CASP-3; Apopain; Protein Yama; SREBP cleavage activity 1; SCA-1. |
| Application Dilution | |
| WB | 1:1000-5000 |
| FCM | FCM1μg/Test |
| IF(IHC-P) | IFIHC-P1:200-800 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is Caspase-3 Monoclonal Antibody, RBITC Conjugated (bsm-55032M-RBITC) validated for, and at what dilution?
bsm-55032M-RBITC is validated for WB(WB) (1:1000-5000); FCM(FCM) (1μg/Test); IF(IHC-P)(IF(IHC-P)) (1:200-800); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Caspase-3 Monoclonal Antibody, RBITC Conjugated (bsm-55032M-RBITC) react with?
Tested reactivity: Human.
How should bsm-55032M-RBITC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Caspase-3 Monoclonal Antibody, RBITC Conjugated (bsm-55032M-RBITC)?
Recombinant human Caspase-3 protein
What are the excitation and emission wavelengths of the RBITC conjugate (bsm-55032M-RBITC)?
RBITC on bsm-55032M-RBITC: excitation / emission 570nm/595nm.