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MUC1/CD227 Recombinant Antibody

25 ug total protein per lane of various lysates (see on figure) probed with MUC1 monoclonal antibody, unconjugated (bsm-55523R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with MUC1 monoclonal antibody, unconjugated (bsm-55523R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC1\/MUC1\/CD227 Monoclonal Antibody, Unconjugated(bsm-55523R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC1\/MUC1\/CD227 Monoclonal Antibody, Unconjugated(bsm-55523R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC1\/MUC1\/CD227 Monoclonal Antibody, Unconjugated(bsm-55523R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC1\/MUC1\/CD227 Monoclonal Antibody, Unconjugated(bsm-55523R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Endometrial; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC1\/MUC1\/CD227 Monoclonal Antibody, Unconjugated(bsm-55523R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Endometrial; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC1\/MUC1\/CD227 Monoclonal Antibody, Unconjugated(bsm-55523R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC1\/MUC1\/CD227 Monoclonal Antibody, Unconjugated(bsm-55523R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC1\/MUC1\/CD227 Monoclonal Antibody, Unconjugated(bsm-55523R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC1\/MUC1\/CD227 Monoclonal Antibody, Unconjugated(bsm-55523R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC1\/MUC1\/CD227 Monoclonal Antibody, Unconjugated(bsm-55523R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC1\/MUC1\/CD227 Monoclonal Antibody, Unconjugated(bsm-55523R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC1\/MUC1\/CD227 Monoclonal Antibody, Unconjugated(bsm-55523R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF(IHC-P)

Reactivity

  • Human
Overview
Catalog # bsm-55523r
Product Name MUC1/CD227 Recombinant Antibody
Applications
WB IHC-P IHC-F IF(IHC-P)
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source Synthesized peptide derived from Human MUC1
Clonality Recombinant
Isotype IgG
Concentration Lot Dependent
Purification Purified by Protein A.
Storage Buffer PBS with 100 μg/ml BSA,0.15% ProClin300 and 50% glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 4582
Swiss Prot P15941
Subcellular location Cytoplasm, Nucleus, Secreted, Cell membrane
Synonyms ADMCKD; ADMCKD1; CD227; MCD; MCKD; MCKD1; PEM; PUM; PEMT; Episialin; EAM; CA 15-3
Application Dilution
WB 1: 500-2000
IHC-P IHC-P1: 200-1000
IHC-F IHC-F1: 200-1000
IF(IHC-P) IFIHC-P1: 200-1000

Frequently asked questions

Which applications is MUC1/CD227 Recombinant Antibody (bsm-55523R) validated for, and at what dilution?

bsm-55523R is validated for WB(WB) (1: 500-2000); IHC-P(IHC-P) (1: 200-1000); IHC-F(IHC-F) (1: 200-1000); IF(IHC-P)(IF(IHC-P)) (1: 200-1000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does MUC1/CD227 Recombinant Antibody (bsm-55523R) react with?

Tested reactivity: Human.

How should bsm-55523R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: PBS with 100 μg/ml BSA,0.15% ProClin300 and 50% glycerol.

What immunogen was used to raise MUC1/CD227 Recombinant Antibody (bsm-55523R)?

Synthesized peptide derived from Human MUC1

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