CD69 Recombinant Antibody

$Daudi (H) treated with PMA (50 ng\/ml) for 24 h, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with CD69 monoclonal antibody, unconjugated (bsm-55564R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

Daudi (H) treated with PMA (50 ng\/ml) for 24 h, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with CD69 monoclonal antibody, unconjugated (bsm-55564R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

$Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD69 Monoclonal Antibody, Unconjugated (bsm-55564R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD69 Monoclonal Antibody, Unconjugated (bsm-55564R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Esophageal Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD69 Monoclonal Antibody, Unconjugated (bsm-55564R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Esophageal Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD69 Monoclonal Antibody, Unconjugated (bsm-55564R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD69 Monoclonal Antibody, Unconjugated (bsm-55564R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD69 Monoclonal Antibody, Unconjugated (bsm-55564R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD69 Monoclonal Antibody, Unconjugated (bsm-55564R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD69 Monoclonal Antibody, Unconjugated (bsm-55564R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Lymph; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD69 Monoclonal Antibody, Unconjugated (bsm-55564R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Lymph; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD69 Monoclonal Antibody, Unconjugated (bsm-55564R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD69 Monoclonal Antibody, Unconjugated (bsm-55564R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD69 Monoclonal Antibody, Unconjugated (bsm-55564R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Applications

WB
ELISA
IHC-F
IP
IF
IHC-P

Reactivity

Human

Overview
Catalog #	bsm-55564r-100ul
Product Name	CD69 Recombinant Antibody
Applications	WB ELISA IHC-F IP IF IHC-P
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CD69
Clonality	Recombinant
Isotype	IgG
Concentration	1mg/ml
Purification	purified by Protein A.
Storage Buffer	0.22μm filtered solution in 20mM hepes7.4, 150mm NaCl, 50% glycerin,0.03% proclin.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	969
Swiss Prot	Q07108
Subcellular location	Cell membrane
Synonyms	AIM; BL-AC/P26; CLEC2C; EA1; GP32/28; MLR-3; 5830438K24Rik; VEA; CD69_HUMAN; CD69; Activation inducer molecule (AIM); C-type lectin domain family 2 member C; Early T-cell activation antigen p60; Leukocyte surface antigen Leu-23; CD69_MOUSE; CD69 molecule; CD69 antigen (p60, early T-cell activation antigen)
Application Dilution
WB	1:500-2000
ELISA	1:5000-20000
IHC-F	1:100-200
IP	1:50-200
IF	1:100-200
IHC-P	1:100-200