DLK1 Recombinant Antibody

$Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with DLK1 Monoclonal Antibody, Unconjugated(bsm-55569R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with DLK1 Monoclonal Antibody, Unconjugated(bsm-55569R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with DLK1 Monoclonal Antibody, Unconjugated(bsm-55569R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with DLK1 Monoclonal Antibody, Unconjugated(bsm-55569R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DLK1 Monoclonal Antibody, Unconjugated (bsm-55569R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DLK1 Monoclonal Antibody, Unconjugated (bsm-55569R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$25 ug total protein per lane of various lysates (see on figure) probed with DLK1 monoclonal antibody, unconjugated (bsm-55569R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with DLK1 monoclonal antibody, unconjugated (bsm-55569R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

IHC-P
IHC-F
IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-55569r
Product Name	DLK1 Recombinant Antibody
Applications	IHC-P IHC-F IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human DLK1
Clonality	Recombinant
Clone #	2A8
Isotype	IgG
Concentration	1mg/ml
Purification	purified by Protein A.
Storage Buffer	0.22μm filtered solution in 20mM hepes7.4, 150mm NaCl, 50% glycerin,0.03% proclin.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	8788
Swiss Prot	P80370
Subcellular location	Cell membrane
Synonyms	DLK; DLK-1; Delta1; FA1; PREF1; Pref-1; ZOG; pG2; DlkI; Ly107; Peg9; SCP1; DLK1_HUMAN; DLK1; DLK1_MOUSE; Adipocyte differentiation inhibitor protein; Preadipocyte factor 1 (Pref-1); Scp-1; delta like non-canonical Notch ligand 1; delta-like homolog (Drosophila); delta-like 1 homolog (Drosophila)
Application Dilution
IHC-P	1:200-400
IHC-F	1:100-500
IF