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DLK1 Recombinant Antibody

Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with DLK1 Monoclonal Antibody, Unconjugated(bsm-55569R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with DLK1 Monoclonal Antibody, Unconjugated(bsm-55569R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with DLK1 Monoclonal Antibody, Unconjugated(bsm-55569R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with DLK1 Monoclonal Antibody, Unconjugated(bsm-55569R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DLK1 Monoclonal Antibody, Unconjugated (bsm-55569R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DLK1 Monoclonal Antibody, Unconjugated (bsm-55569R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
25 ug total protein per lane of various lysates (see on figure) probed with DLK1 monoclonal antibody, unconjugated (bsm-55569R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with DLK1 monoclonal antibody, unconjugated (bsm-55569R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-F
  • IF
  • IHC-P

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-55569r
Product Name DLK1 Recombinant Antibody
Applications
WB IHC-F IF IHC-P
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human DLK1
Clonality Recombinant
Clone # 2A8
Isotype IgG
Concentration Lot Dependent
Purification purified by Protein A.
Storage Buffer 0.22μm filtered solution in 20mM hepes7.4, 150mm NaCl, 50% glycerin,0.03% proclin.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 8788
Swiss Prot P80370
Subcellular location Cell membrane
Synonyms DLK; DLK-1; Delta1; FA1; PREF1; Pref-1; ZOG; pG2; DlkI; Ly107; Peg9; SCP1; DLK1_HUMAN; DLK1; DLK1_MOUSE; Adipocyte differentiation inhibitor protein; Preadipocyte factor 1 (Pref-1); Scp-1; delta like non-canonical Notch ligand 1; delta-like homolog (Drosophila); delta-like 1 homolog (Drosophila)
Application Dilution
WB 1:500-2000
IHC-F 1:100-500
IF 1:100-500
IHC-P 1:100-500

Frequently asked questions

Which applications is DLK1 Recombinant Antibody (bsm-55569R) validated for, and at what dilution?

bsm-55569R is validated for WB (1:500-2000); IHC-F (1:100-500); IF (1:100-500); IHC-P (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does DLK1 Recombinant Antibody (bsm-55569R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-55569R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.22μm filtered solution in 20mM hepes7.4, 150mm NaCl, 50% glycerin,0.03% proclin.

What immunogen was used to raise DLK1 Recombinant Antibody (bsm-55569R)?

KLH conjugated synthetic peptide derived from human DLK1

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