PLAUR Recombinant Antibody

$Paraformaldehyde-fixed, paraffin embedded Human lung squamous cell carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PLAUR Monoclonal Antibody, Unconjugated (bsm-55595R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human lung squamous cell carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PLAUR Monoclonal Antibody, Unconjugated (bsm-55595R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PLAUR Monoclonal Antibody, Unconjugated (bsm-55595R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PLAUR Monoclonal Antibody, Unconjugated (bsm-55595R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PLAUR Monoclonal Antibody, Unconjugated (bsm-55595R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PLAUR Monoclonal Antibody, Unconjugated (bsm-55595R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PLAUR Monoclonal Antibody, Unconjugated (bsm-55595R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PLAUR Monoclonal Antibody, Unconjugated (bsm-55595R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PLAUR Monoclonal Antibody, Unconjugated (bsm-55595R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PLAUR Monoclonal Antibody, Unconjugated (bsm-55595R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Kideny; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PLAUR Monoclonal Antibody, Unconjugated (bsm-55595R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Kideny; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PLAUR Monoclonal Antibody, Unconjugated (bsm-55595R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$U-937 (H) cells were treated with or without PMA (200 nM) for 72 h, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with PLAUR monoclonal antibody, unconjugated (bsm-55595R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

U-937 (H) cells were treated with or without PMA (200 nM) for 72 h, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with PLAUR monoclonal antibody, unconjugated (bsm-55595R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
IHC-P
IHC-F
IF

Reactivity

Human

Predicted Reactivity

Mouse

Overview
Catalog #	bsm-55595r
Product Name	PLAUR Recombinant Antibody
Applications	WB, IHC-P, IHC-F, IF
Reactivity	Human
Predicted Reactivity	Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant human PLAUR protein
Clonality	Recombinant
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Swiss Prot	Q03405
Subcellular location	Secreted, Cell membrane
Synonyms	Plasminogen activator, urokinase receptor; Urokinase plasminogen activator surface receptor; Monocyte activation antigen Mo3; U-PAR; U PAR; UPAR; MO3; MO 3; CD 87; CD87; Monocyte activation antigen Mo3; u plasminogen activator receptor; UPA receptor; Urinary plasminogen activator receptor; URKR; Urokinase plasminogen activator receptor;
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	1:100-500
IF