SOX2 Monoclonal Antibody

$The NCCIT (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Mouse Anti-SOX2 antibody (bsm-55601M,1:100); Isotype Control (orange): Mouse IgG (bs-0296P). Blank control (black): PBS. Acquisition of 20,000 events was performed.$

The NCCIT (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Mouse Anti-SOX2 antibody (bsm-55601M,1:100); Isotype Control (orange): Mouse IgG (bs-0296P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

Applications

IHC-P
IHC-F
IF
Flow-Cyt

Reactivity

Human
Rat

Predicted Reactivity

Mouse

Overview
Catalog #	bsm-55601m
Product Name	SOX2 Monoclonal Antibody
Applications	IHC-P IHC-F IF FLOW-CYT
Reactivity	Human, Rat
Predicted Reactivity	Mouse
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	KLH conjugated synthetic peptide derived from human SOX2
Clonality	Monoclonal
Clone #	7A4
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Swiss Prot	P48431
Subcellular location	Nucleus
Synonyms	ANOP3; MCOPS3; Sox-2; lcc; ysb; SOX2_HUMAN; SOX2; SOX2_MOUSE;
Application Dilution
IHC-P	=1:50-200
IHC-F	IHC-F=1:50-200
IF	IF=1:50-200
Flow-Cyt	Flow-Cyt=1:50-100