OCLN | Occludin Recombinant Antibody

$Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OCLN | Occludin Monoclonal Antibody, Unconjugated (bsm-55617R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OCLN | Occludin Monoclonal Antibody, Unconjugated (bsm-55617R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OCLN | Occludin Monoclonal Antibody, Unconjugated (bsm-55617R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OCLN | Occludin Monoclonal Antibody, Unconjugated (bsm-55617R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OCLN | Occludin Monoclonal Antibody, Unconjugated (bsm-55617R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OCLN | Occludin Monoclonal Antibody, Unconjugated (bsm-55617R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OCLN | Occludin Monoclonal Antibody, Unconjugated (bsm-55617R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OCLN | Occludin Monoclonal Antibody, Unconjugated (bsm-55617R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OCLN | Occludin Monoclonal Antibody, Unconjugated (bsm-55617R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OCLN | Occludin Monoclonal Antibody, Unconjugated (bsm-55617R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OCLN | Occludin Monoclonal Antibody, Unconjugated (bsm-55617R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OCLN | Occludin Monoclonal Antibody, Unconjugated (bsm-55617R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OCLN | Occludin Monoclonal Antibody, Unconjugated (bsm-55617R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OCLN | Occludin Monoclonal Antibody, Unconjugated (bsm-55617R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OCLN | Occludin Monoclonal Antibody, Unconjugated (bsm-55617R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OCLN | Occludin Monoclonal Antibody, Unconjugated (bsm-55617R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OCLN | Occludin Monoclonal Antibody, Unconjugated (bsm-55617R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OCLN | Occludin Monoclonal Antibody, Unconjugated (bsm-55617R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OCLN | Occludin Monoclonal Antibody, Unconjugated (bsm-55617R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OCLN | Occludin Monoclonal Antibody, Unconjugated (bsm-55617R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Clear cell carcinoma of ovary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OCLN | Occludin Monoclonal Antibody, Unconjugated (bsm-55617R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Clear cell carcinoma of ovary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OCLN | Occludin Monoclonal Antibody, Unconjugated (bsm-55617R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OCLN | Occludin Monoclonal Antibody, Unconjugated (bsm-55617R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OCLN | Occludin Monoclonal Antibody, Unconjugated (bsm-55617R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.

Applications

WB
ELISA
IHC-F
IP
IF
IHC-P

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-55617r-100ul
Product Name	OCLN \| Occludin Recombinant Antibody
Applications	WB ELISA IHC-F IP IF IHC-P
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant human Occludin protein
Clonality	Recombinant
Clone #	8F3
Concentration	1mg/ml
Purification	purified by Protein A.
Storage Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	100506658
Swiss Prot	Q61146
Subcellular location	Cell membrane
Synonyms	Ocl; BLCPMG; PPP1R115; PTORCH1; OCLN_CANLF; OCLN; OCLN_HUMAN; OCLN_MOUSE; OCLN_RAT; occludin; tight junction protein occludin TM4 minus; phosphatase 1, regulatory subunit 115
Application Dilution
WB	1:2000-10000
ELISA	1:5000-20000
IHC-F	1:100-500
IP	1:50-200
IF	1:100-500
IHC-P	1:100-500