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TUBA4A | alpha-Tubulin Recombinant Antibody

4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (alpha Tubulin) monoclonal Antibody, unconjugated (bsm-55636R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (alpha Tubulin) monoclonal Antibody, unconjugated (bsm-55636R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
25 ug total protein per lane of various lysates (see on figure) probed with TUBA4A | alpha-Tubulin monoclonal antibody, unconjugated (bsm-55636R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with TUBA4A | alpha-Tubulin monoclonal antibody, unconjugated (bsm-55636R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TUBA4A | alpha-Tubulin Monoclonal Antibody, Unconjugated (bsm-55636R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TUBA4A | alpha-Tubulin Monoclonal Antibody, Unconjugated (bsm-55636R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TUBA4A | alpha-Tubulin Monoclonal Antibody, Unconjugated (bsm-55636R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TUBA4A | alpha-Tubulin Monoclonal Antibody, Unconjugated (bsm-55636R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TUBA4A | alpha-Tubulin Monoclonal Antibody, Unconjugated (bsm-55636R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TUBA4A | alpha-Tubulin Monoclonal Antibody, Unconjugated (bsm-55636R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TUBA4A | alpha-Tubulin Monoclonal Antibody, Unconjugated (bsm-55636R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TUBA4A | alpha-Tubulin Monoclonal Antibody, Unconjugated (bsm-55636R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TUBA4A | alpha-Tubulin Monoclonal Antibody, Unconjugated (bsm-55636R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TUBA4A | alpha-Tubulin Monoclonal Antibody, Unconjugated (bsm-55636R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TUBA4A | alpha-Tubulin Monoclonal Antibody, Unconjugated (bsm-55636R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TUBA4A | alpha-Tubulin Monoclonal Antibody, Unconjugated (bsm-55636R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TUBA4A | alpha-Tubulin Monoclonal Antibody, Unconjugated (bsm-55636R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TUBA4A | alpha-Tubulin Monoclonal Antibody, Unconjugated (bsm-55636R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human right temporal lobe; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TUBA4A | alpha-Tubulin Monoclonal Antibody, Unconjugated (bsm-55636R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human right temporal lobe; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TUBA4A | alpha-Tubulin Monoclonal Antibody, Unconjugated (bsm-55636R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TUBA4A | alpha-Tubulin Monoclonal Antibody, Unconjugated (bsm-55636R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TUBA4A | alpha-Tubulin Monoclonal Antibody, Unconjugated (bsm-55636R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
The HeLa (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-alpha Tubulin antibody (bsm-55636R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
The HeLa (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-alpha Tubulin antibody (bsm-55636R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
Bioss unconjugated antibody vial

Applications

  • WB
  • ELISA
  • IHC-F
  • IP
  • IF
  • IHC-P
  • Flow-Cyt
  • ICC/IF

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-55636r-100uL
Product Name TUBA4A | alpha-Tubulin Recombinant Antibody
Applications
WB ELISA IHC-F IP IF IHC-P FLOW-CYT ICC/IF
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human TUBA4A
Clonality Recombinant
Isotype IgG
Concentration 1mg/ml
Purification purified by Protein A.
Storage Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 7277
Swiss Prot P68366
Subcellular location Cytoplasm
Synonyms ALS22; CMYO26; FTDALS9; H2-ALPHA; OZEMA23; SPAX11; TUBA1; M[a]4; Tuba4; TBA4A_HUMAN; TUBA4A; Alpha-tubulin 1; Testis-specific alpha-tubulin; Tubulin H2-alpha; Tubulin alpha-1 chain; 3.6.5.-; TBA4A_MOUSE; Alpha-tubulin 4; Alpha-tubulin isotype M-alpha-4; Tubulin alpha-4 chain; TBA4A_RAT; tubulin alpha 4a; tubulin, alpha 1 (testis specific); tubulin, alpha 1
Application Dilution
WB 1:1000-2000
ELISA 1:5000-10000
IHC-F 1:200-800
IP 1:50-100
IF 1:200-800
IHC-P 1:200-800
Flow-Cyt 1:100-200
ICC/IF 1:100-200

Frequently asked questions

Which applications is TUBA4A | alpha-Tubulin Recombinant Antibody (bsm-55636R) validated for, and at what dilution?

bsm-55636R is validated for WB (1:1000-2000); ELISA (1:5000-10000); IHC-F (1:200-800); IP (1:50-100); IF (1:200-800); IHC-P (1:200-800); Flow-Cyt (1:100-200); ICC/IF (1:100-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does TUBA4A | alpha-Tubulin Recombinant Antibody (bsm-55636R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-55636R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise TUBA4A | alpha-Tubulin Recombinant Antibody (bsm-55636R)?

KLH conjugated synthetic peptide derived from human TUBA4A

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