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TGF Beta 1/3 Recombinant Antibody

25 ug total protein per lane of various lysates (see on figure) probed with TGF Beta 1\/3 monoclonal antibody, unconjugated (bsm-55638R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with TGF Beta 1\/3 monoclonal antibody, unconjugated (bsm-55638R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Embryo; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TGF Beta 1\/3 Monoclonal Antibody, Unconjugated (bsm-55638R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Embryo; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TGF Beta 1\/3 Monoclonal Antibody, Unconjugated (bsm-55638R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Embryo; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TGF Beta 1\/3 Monoclonal Antibody, Unconjugated (bsm-55638R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Embryo; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TGF Beta 1\/3 Monoclonal Antibody, Unconjugated (bsm-55638R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TGF Beta 1\/3 Monoclonal Antibody, Unconjugated (bsm-55638R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TGF Beta 1\/3 Monoclonal Antibody, Unconjugated (bsm-55638R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TGF Beta 1\/3 Monoclonal Antibody, Unconjugated (bsm-55638R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TGF Beta 1\/3 Monoclonal Antibody, Unconjugated (bsm-55638R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TGF Beta 1\/3 Monoclonal Antibody, Unconjugated (bsm-55638R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TGF Beta 1\/3 Monoclonal Antibody, Unconjugated (bsm-55638R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • WB
  • ELISA

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-55638r-100ul
Product Name TGF Beta 1/3 Recombinant Antibody
Applications
WB ELISA
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source LH conjugated synthetic peptide derived from human TGF Beta 1
Clonality Recombinant
Clone # 4H12
Isotype IgG
Concentration Lot Dependent
Purification purified by Protein A.
Storage Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 7040
Swiss Prot P01137
Subcellular location Secreted, Extracellular matrix
Application Dilution
WB =1:500-2000
ELISA ELISA=1:5000-10000

Frequently asked questions

Which applications is TGF Beta 1/3 Recombinant Antibody (bsm-55638R) validated for, and at what dilution?

bsm-55638R is validated for WB (WB=1:500-2000); ELISA (ELISA=1:5000-10000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does TGF Beta 1/3 Recombinant Antibody (bsm-55638R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-55638R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise TGF Beta 1/3 Recombinant Antibody (bsm-55638R)?

LH conjugated synthetic peptide derived from human TGF Beta 1

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