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CYP11A1 Recombinant Antibody

25 ug total protein per lane of various lysates (see on figure) probed with CYP11A1 monoclonal antibody, unconjugated (bsm-55673R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with CYP11A1 monoclonal antibody, unconjugated (bsm-55673R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP11A1 Monoclonal Antibody, Unconjugated (bsm-55673R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP11A1 Monoclonal Antibody, Unconjugated (bsm-55673R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP11A1 Monoclonal Antibody, Unconjugated (bsm-55673R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP11A1 Monoclonal Antibody, Unconjugated (bsm-55673R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP11A1 Monoclonal Antibody, Unconjugated (bsm-55673R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP11A1 Monoclonal Antibody, Unconjugated (bsm-55673R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Ovary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP11A1 Monoclonal Antibody, Unconjugated (bsm-55673R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Ovary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP11A1 Monoclonal Antibody, Unconjugated (bsm-55673R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Ovary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP11A1 Monoclonal Antibody, Unconjugated (bsm-55673R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Ovary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP11A1 Monoclonal Antibody, Unconjugated (bsm-55673R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Ovary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP11A1 Monoclonal Antibody, Unconjugated (bsm-55673R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Ovary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP11A1 Monoclonal Antibody, Unconjugated (bsm-55673R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Adrenal Gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP11A1 Monoclonal Antibody, Unconjugated (bsm-55673R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Adrenal Gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP11A1 Monoclonal Antibody, Unconjugated (bsm-55673R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Adrenal Gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP11A1 Monoclonal Antibody, Unconjugated (bsm-55673R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Adrenal Gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP11A1 Monoclonal Antibody, Unconjugated (bsm-55673R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Adrenal Gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP11A1 Monoclonal Antibody, Unconjugated (bsm-55673R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Adrenal Gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP11A1 Monoclonal Antibody, Unconjugated (bsm-55673R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP11A1 Monoclonal Antibody, Unconjugated (bsm-55673R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP11A1 Monoclonal Antibody, Unconjugated (bsm-55673R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP11A1 Monoclonal Antibody, Unconjugated (bsm-55673R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP11A1 Monoclonal Antibody, Unconjugated (bsm-55673R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • WB
  • ELISA
  • IHC-F
  • IP
  • IF
  • IHC-P

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-55673r-100ul
Product Name CYP11A1 Recombinant Antibody
Applications
WB ELISA IHC-F IP IF IHC-P
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CYP11A1
Clonality Recombinant
Clone # 12D1
Isotype IgG
Concentration Lot Dependent
Purification purified by Protein A.
Storage Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 1583
Swiss Prot P05108
Subcellular location Cytoplasm
Synonyms CYP11A; CYPXIA1; P450SCC; D9Ertd411e; Scc; cscc; P450(scc); CP11A_HUMAN; CYP11A1; Cholesterol desmolase; Cytochrome P450 11A1; Cytochrome P450(scc); 1.14.15.6; CP11A_MOUSE; CP11A_RAT; Cyp11a-1; cytochrome P450 family 11 subfamily A member 1; cytochrome P450, subfamily XIA (cholesterol side chain cleavage); cytochrome P450, family 11, subfamily A, polypeptide 1; cholesterol monooxygenase (side-chain-cleaving)
Application Dilution
WB 1:1000-2000
ELISA 1:5000-10000
IHC-F 1:100-500
IP 1:50-100
IF 1:100-500
IHC-P 1:100-500

Frequently asked questions

Which applications is CYP11A1 Recombinant Antibody (bsm-55673R) validated for, and at what dilution?

bsm-55673R is validated for WB (1:1000-2000); ELISA (1:5000-10000); IHC-F (1:100-500); IP (1:50-100); IF (1:100-500); IHC-P (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CYP11A1 Recombinant Antibody (bsm-55673R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-55673R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CYP11A1 Recombinant Antibody (bsm-55673R)?

KLH conjugated synthetic peptide derived from human CYP11A1

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