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BrdU Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded BrdU-treated Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with BrdU Monoclonal Antibody, Unconjugated (bsm-55724M) at 1:500 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded BrdU-treated Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with BrdU Monoclonal Antibody, Unconjugated (bsm-55724M) at 1:500 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded BrdU-treated Mouse Jejunum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with BrdU Monoclonal Antibody, Unconjugated (bsm-55724M) at 1:500 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded BrdU-treated Mouse Jejunum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with BrdU Monoclonal Antibody, Unconjugated (bsm-55724M) at 1:500 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded BrdU-treated Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with BrdU Monoclonal Antibody, Unconjugated (bsm-55724M) at 1:500 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded BrdU-treated Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with BrdU Monoclonal Antibody, Unconjugated (bsm-55724M) at 1:500 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded BrdU-treated Mouse Duodenum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with BrdU Monoclonal Antibody, Unconjugated (bsm-55724M) at 1:500 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded BrdU-treated Mouse Duodenum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with BrdU Monoclonal Antibody, Unconjugated (bsm-55724M) at 1:500 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded BrdU-treated Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with BrdU Monoclonal Antibody, Unconjugated (bsm-55724M) at 1:500 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded BrdU-treated Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with BrdU Monoclonal Antibody, Unconjugated (bsm-55724M) at 1:500 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded BrdU-treated Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with BrdU Monoclonal Antibody, Unconjugated (bsm-55724M) at 1:500 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded BrdU-treated Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with BrdU Monoclonal Antibody, Unconjugated (bsm-55724M) at 1:500 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • IHC-F
  • IF
  • IHC-P

Reactivity

  • Species independent
Overview
Catalog # bsm-55724m-100uL
Product Name BrdU Monoclonal Antibody
Applications
IHC-F IF IHC-P
Reactivity Species independent
Specifications
Conjugation Unconjugated
Host Mouse
Source KLH conjugated Brdu
Clonality Monoclonal
Isotype IgG1, k
Concentration Lot Dependent
Purification purified by Protein A.
Storage Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Subcellular location Nucleus
Synonyms Bromodeoxyuridine; Bromodeoxyuridine; 5-Bromo-2′-deoxyuridine; 5-BrdU; Proliferation Marker; 5-Bromo-2-deoxyUridine.;
Application Dilution
IHC-F 1:200-1000
IF 1:200-1000
IHC-P 1:200-1000

Frequently asked questions

Which applications is BrdU Monoclonal Antibody (bsm-55724M) validated for, and at what dilution?

bsm-55724M is validated for IHC-F (1:200-1000); IF (1:200-1000); IHC-P (1:200-1000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does BrdU Monoclonal Antibody (bsm-55724M) react with?

Tested reactivity: Species independent.

How should bsm-55724M be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise BrdU Monoclonal Antibody (bsm-55724M)?

KLH conjugated Brdu

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