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MEK1/2 (Ser217/Ser221) Recombinant Antibody

Jurkat (H) serum-starved for 16 h, then treated with TPA (200 nM) for 30 min, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with phospho-MEK1\/2 (Ser217\/Ser221) monoclonal antibody, unconjugated (bsm-55741R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Jurkat (H) serum-starved for 16 h, then treated with TPA (200 nM) for 30 min, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with phospho-MEK1\/2 (Ser217\/Ser221) monoclonal antibody, unconjugated (bsm-55741R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-MEK1\/2 (Ser217\/Ser221) Monoclonal Antibody, Unconjugated (bsm-55741R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-MEK1\/2 (Ser217\/Ser221) Monoclonal Antibody, Unconjugated (bsm-55741R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-MEK1\/2 (Ser217\/Ser221) Monoclonal Antibody, Unconjugated (bsm-55741R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-MEK1\/2 (Ser217\/Ser221) Monoclonal Antibody, Unconjugated (bsm-55741R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • WB
  • ELISA
  • IHC-F
  • IP
  • IF
  • IHC-P

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
Overview
Catalog # bsm-55741r-100ul
Product Name MEK1/2 (Ser217/Ser221) Recombinant Antibody
Applications
WB ELISA IHC-F IP IF IHC-P
Reactivity Human
Predicted Reactivity Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated Synthesised phosphopeptide derived from human MEK1 around the phosphorylation site of Ser217/221
Clonality Recombinant
Clone # 12C3
Isotype IgG
Concentration Lot Dependent
Purification purified by Protein A.
Storage Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Swiss Prot Q02750
Synonyms MEK1 & MEK2 (phospho-S218/S222); p-MEK1 & MEK2; phospho-MEK1 & MEK2; MEK1 & MEK2 (phospho-Ser218/Ser222); CFC4; MAPKK2; MEK2; MKK2; PRKMK2; MK2; MP2K2_HUMAN; MAP2K2; MAP kinase kinase 2; MAPKK 2; ERK activator kinase 2; MAPK/ERK kinase 2 (MEK 2); 2.7.12.2
Application Dilution
WB 1:500-2000
ELISA 1:5000-10000
IHC-F 1:50-200
IP 1:50-200
IF 1:50-200
IHC-P 1:50-200

Frequently asked questions

Which applications is MEK1/2 (Ser217/Ser221) Recombinant Antibody (bsm-55741R) validated for, and at what dilution?

bsm-55741R is validated for WB (1:500-2000); ELISA (1:5000-10000); IHC-F (1:50-200); IP (1:50-200); IF (1:50-200); IHC-P (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does MEK1/2 (Ser217/Ser221) Recombinant Antibody (bsm-55741R) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.

How should bsm-55741R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise MEK1/2 (Ser217/Ser221) Recombinant Antibody (bsm-55741R)?

KLH conjugated Synthesised phosphopeptide derived from human MEK1 around the phosphorylation site of Ser217/221

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