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CHI3L1 Recombinant Antibody

THP-1 (H) treated with PMA (100 ng\/mL) for 24 h, Replace with fresh complete culture medium and continue to culture for 24 h, + hIFN-\u03b1 (500U\/mL) for 24 h, then with Brefeldin A (1\u00b5g\/ml) for 3 h, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with CHI3L1 monoclonal antibody, unconjugated (bsm-55783R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
THP-1 (H) treated with PMA (100 ng\/mL) for 24 h, Replace with fresh complete culture medium and continue to culture for 24 h, + hIFN-\u03b1 (500U\/mL) for 24 h, then with Brefeldin A (1\u00b5g\/ml) for 3 h, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with CHI3L1 monoclonal antibody, unconjugated (bsm-55783R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Lung squamous cell carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CHI3L1 Monoclonal Antibody, Unconjugated (bsm-55783R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung squamous cell carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CHI3L1 Monoclonal Antibody, Unconjugated (bsm-55783R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CHI3L1 Monoclonal Antibody, Unconjugated (bsm-55783R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CHI3L1 Monoclonal Antibody, Unconjugated (bsm-55783R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CHI3L1 Monoclonal Antibody, Unconjugated (bsm-55783R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CHI3L1 Monoclonal Antibody, Unconjugated (bsm-55783R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CHI3L1 Monoclonal Antibody, Unconjugated (bsm-55783R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CHI3L1 Monoclonal Antibody, Unconjugated (bsm-55783R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CHI3L1 Monoclonal Antibody, Unconjugated (bsm-55783R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CHI3L1 Monoclonal Antibody, Unconjugated (bsm-55783R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CHI3L1 Monoclonal Antibody, Unconjugated (bsm-55783R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CHI3L1 Monoclonal Antibody, Unconjugated (bsm-55783R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • IHC-F
  • IF
  • IHC-P

Reactivity

  • Human
Overview
Catalog # bsm-55783r-100uL
Product Name CHI3L1 Recombinant Antibody
Applications
IHC-F IF IHC-P
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CHI3L1
Clonality Recombinant
Isotype IgG
Concentration Lot Dependent
Purification purified by Protein A.
Storage Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 1116
Swiss Prot P36222
Subcellular location Secreted, Extracellular matrix
Synonyms ASRT7; CGP-39; GP-39; GP39; HC-gp39; HCGP-3P; YK-40; YKL-40; YKL40; YYL-40; hCGP-39; Brp39; Chil1; GP38K; CH3L1_HUMAN; CHI3L1; 39 kDa synovial protein; Cartilage glycoprotein 39 (CGP-39 | GP-39 | hCGP-39); CH3L1_MOUSE; BRP39 protein; Breast regression protein 39; Cartilage glycoprotein 39 (CGP-39 | GP-39); CH3L1_PONAB; CH3L1_PIG; 38 kDa heparin-binding glycoprotein; Signal-processing protein; CH3L1_RAT; chitinase 3 like 1; chitinase 3-like 1 (cartilage glycoprotein-39); cartilage glycoprotein-39;
Application Dilution
IHC-F 1:50-200
IF 1:50-200
IHC-P 1:50-200

Frequently asked questions

Which applications is CHI3L1 Recombinant Antibody (bsm-55783R) validated for, and at what dilution?

bsm-55783R is validated for IHC-F (1:50-200); IF (1:50-200); IHC-P (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CHI3L1 Recombinant Antibody (bsm-55783R) react with?

Tested reactivity: Human.

How should bsm-55783R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CHI3L1 Recombinant Antibody (bsm-55783R)?

KLH conjugated synthetic peptide derived from human CHI3L1

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US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

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