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H2AX | Histone H2A.X (ub-K119) Recombinant Antibody

Applications

  • WB
  • ELISA
  • IHC-F
  • IP
  • IF
  • ChIP
  • IHC-P

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-55852r-100ul
Product Name H2AX | Histone H2A.X (ub-K119) Recombinant Antibody
Applications
WB ELISA IHC-F IP IF CHIP IHC-P
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated Synthesised Ubiquitylpeptide derived from human H2AX around the Ubiquitylation site of Lys119
Clonality Recombinant
Isotype IgG
Concentration 1mg/ml
Purification purified by Protein A.
Storage Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 3014
Swiss Prot P16104
Subcellular location Nucleus
Synonyms H2A.X; H2A/X; H2AFX; Hist5-2ax; gammaH2ax; RGD1566119; H2AX_HUMAN; H2AX; Histone H2A.X; H2AX_MOUSE; H2A.X variant histone; H2A histone family, member X; H2A histone family member X; H2AX (ubiquityl-K119); H2AXK119Ub; ub-H2AX; ubiquityl-H2AX; H2AX (ubiquityl-Lys119)
Application Dilution
WB 1:500-2000
ELISA 1:5000-20000
IHC-F 1:200-800
IP 1:50-200
IF 1:200-800
ChIP 1:50-100
IHC-P 1:200-800

Frequently asked questions

Which applications is H2AX | Histone H2A.X (ub-K119) Recombinant Antibody (bsm-55852R) validated for, and at what dilution?

bsm-55852R is validated for WB (1:500-2000); ELISA (1:5000-20000); IHC-F (1:200-800); IP (1:50-200); IF (1:200-800); ChIP (1:50-100); IHC-P (1:200-800). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does H2AX | Histone H2A.X (ub-K119) Recombinant Antibody (bsm-55852R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-55852R be stored?

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise H2AX | Histone H2A.X (ub-K119) Recombinant Antibody (bsm-55852R)?

KLH conjugated Synthesised Ubiquitylpeptide derived from human H2AX around the Ubiquitylation site of Lys119