IKB alpha Monoclonal Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-56204r-rbitc-100uL |
| Product Name | IKB alpha Monoclonal Antibody, RBITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NFKBIA |
| Clonality | Monoclonal |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4792 |
| Swiss Prot | P25963 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | NFKBIA; Inhibitor of KB alpha; I kappa B alpha; I(Kappa)B(alpha); IkappaBalpha; IKBA; IKBalpha; MAD 3; MAD3; Major histocompatibility complex enhancer binding protein MAD3; NF kappa B inhibitor alpha; NFKBI; Nuclear factor of kappa light chain gene enhancer in B cells; Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha; IKBA_HUMAN; IκB-α; IκBα. |
| Background | Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription. |
| Application Dilution | |
| WB | IFICC1:50-200 |
| IF(IHC-P) | IHC-P1:50-200 |
| IF(ICC) | WB1:500-2000 |
Frequently asked questions
Which applications is IKB alpha Monoclonal Antibody, RBITC Conjugated (bsm-56204R-RBITC) validated for, and at what dilution?
bsm-56204R-RBITC is validated for WB(IF(ICC)) (1:50-200); IF(IHC-P)(IHC-P) (1:50-200); IF(ICC)(WB) (1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does IKB alpha Monoclonal Antibody, RBITC Conjugated (bsm-56204R-RBITC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-56204R-RBITC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise IKB alpha Monoclonal Antibody, RBITC Conjugated (bsm-56204R-RBITC)?
KLH conjugated synthetic peptide derived from human NFKBIA
What are the excitation and emission wavelengths of the RBITC conjugate (bsm-56204R-RBITC)?
RBITC on bsm-56204R-RBITC: excitation / emission 570nm/595nm.