Myogenin Recombinant Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-56206r-cy3-100uL |
| Product Name | Myogenin Recombinant Antibody, Cy3 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Myogenin |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4656 |
| Swiss Prot | P15173 |
| Subcellular location | Nucleus |
| Synonyms | MYOG_HUMAN; MYOG_MOUSE; MYOG; BHLHC3; MYF4; Class C basic helix-loop-helix protein 3 (bHLHc3); Myogenic factor 4 (Myf-4); BHLHC3; MYF4; MYF 4 |
| Background | Myogenin is a member of a family of myogenic regulatory genes, which includes MyoD, myf5 and MRF4. These genes encode a set of transcription factors, which are essential for muscle development. Transfection of myogenin into multipotential mesodermal cells have been shown to convert the mesodermal cells to myoblasts. Expression of myogenin is restricted to cells of skeletal muscle origin. It is therefore a useful marker for tumors of the muscle lineage, being strongly expressed in alveolar rhabdomyosarcomas. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:50-200 |
Frequently asked questions
Which applications is Myogenin Recombinant Antibody, Cy3 Conjugated (bsm-56206R-Cy3) validated for, and at what dilution?
bsm-56206R-Cy3 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Myogenin Recombinant Antibody, Cy3 Conjugated (bsm-56206R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-56206R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Myogenin Recombinant Antibody, Cy3 Conjugated (bsm-56206R-Cy3)?
KLH conjugated synthetic peptide derived from human Myogenin
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-56206R-Cy3)?
Cy3 on bsm-56206R-Cy3: excitation / emission 512,550nm/570,615nm.