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KLK8 Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (KLK8) Monoclonal Antibody, Unconjugated (bsm-56216M) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (KLK8) Monoclonal Antibody, Unconjugated (bsm-56216M) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human Pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (KLK8) Monoclonal Antibody, Unconjugated (bsm-56216M) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human Pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (KLK8) Monoclonal Antibody, Unconjugated (bsm-56216M) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Bioss unconjugated antibody vial

Applications

  • IHC

Reactivity

  • Human
Overview
Catalog # bsm-56216m
Product Name KLK8 Monoclonal Antibody
Applications
IHC
Reactivity Human
Specifications
Conjugation Unconjugated
Host Mouse
Source Recombinant protein of human KLK8
Clonality Monoclonal
Clone # 6B13
Isotype IgG2b
Concentration Lot Dependent
Purification purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 11202
Swiss Prot Q60259
Subcellular location Cytoplasm, Secreted
Synonyms Kallikrein-8
Background

Serine protease which is capable of degrading a number of proteins such as casein, fibrinogen, kininogen, fibronectin and collagen type IV. Also cleaves L1CAM in response to increased neural activity. Induces neurite outgrowth and fasciculation of cultured hippocampal neurons. Plays a role in the formation and maturation of orphan and small synaptic boutons in the Schaffer-collateral pathway, regulates Schaffer-collateral long-term potentiation in the hippocampus and is required for memory acquisition and synaptic plasticity. Involved in skin desquamation and keratinocyte proliferation. Plays a role in the secondary phase of pathogenesis following spinal cord injury.
Application Dilution
IHC 1:100-500

Frequently asked questions

Which applications is KLK8 Monoclonal Antibody (bsm-56216M) validated for, and at what dilution?

bsm-56216M is validated for IHC (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does KLK8 Monoclonal Antibody (bsm-56216M) react with?

Tested reactivity: Human.

How should bsm-56216M be stored?

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise KLK8 Monoclonal Antibody (bsm-56216M)?

Recombinant protein of human KLK8

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