PMP22 Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in EDTA buffer (Ph9.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (PMP22) Monoclonal Antibody, Unconjugated (bsm-56226M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in EDTA buffer (Ph9.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (PMP22) Monoclonal Antibody, Unconjugated (bsm-56226M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

$Paraformaldehyde-fixed, paraffin embedded (human stomach); Antigen retrieval by boiling in EDTA buffer (Ph9.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (PMP22) Monoclonal Antibody, Unconjugated (bsm-56226M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (human stomach); Antigen retrieval by boiling in EDTA buffer (Ph9.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (PMP22) Monoclonal Antibody, Unconjugated (bsm-56226M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

$Paraformaldehyde-fixed, paraffin embedded (Human Fundus); Antigen retrieval by boiling in EDTA buffer (Ph9.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (PMP22) Monoclonal Antibody, Unconjugated (bsm-56226M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (Human Fundus); Antigen retrieval by boiling in EDTA buffer (Ph9.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (PMP22) Monoclonal Antibody, Unconjugated (bsm-56226M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Applications

Reactivity

Human

Predicted Reactivity

Mouse
Rat

Overview
Catalog #	bsm-56226m
Product Name	PMP22 Monoclonal Antibody
Applications	IHC
Reactivity	Human
Predicted Reactivity	Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant protein of human PMP22
Clonality	Monoclonal
Clone #	2F7
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5376
Swiss Prot	Q01453
Subcellular location	Cell membrane
Synonyms	GAS3; CMT1A; CMT1E; DSS; GAS-3; Growth Arrest Specific 3; Growth arrest-specific protein 3; HMSNIA; HNPP; MGC20769; Peripheral Myelin Protein 22; PMP-22; PMP22; PMP22_HUMAN; Sp110; Trembler.
Background	PMP22 is a 22 kDa glycoprotein expressed in the compact myelin of the peripheral nervous system. In the peripheral nervous system, PMP 22 is produced by myelinating Schwann cells and is coexpressed with the genes for myelin basic protein (MBP) during nerve development and regeneration. Alterations in the level of this protein cause several genetic human diseases. If the protein is duplicated, patients develop Charcot Marie Tooth disease. If one copy of the gene is deleted, they suffer from the inherited tendency to pressure palsies.
Application Dilution
IHC	1:100-500