ROCK2 Monoclonal Antibody

$Lane 1: Human HepG2 cell lysates; Lane 2: Human Hela cell lysates probed with ROCK2 Monoclonal Antibody, Unconjugated (bsm-60013M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: Human HepG2 cell lysates; Lane 2: Human Hela cell lysates probed with ROCK2 Monoclonal Antibody, Unconjugated (bsm-60013M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

Applications

Reactivity

Human

Predicted Reactivity

Mouse

Overview
Catalog #	bsm-60013M
Product Name	ROCK2 Monoclonal Antibody
Applications	WB
Reactivity	Human
Predicted Reactivity	Mouse
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	KLH conjugated synthetic peptide derived from human ROCK2
Clonality	Monoclonal
Clone #	E2C11
Isotype	IgG
Concentration	Lot Dependent
Purification	purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	9475
Swiss Prot	O75116
Subcellular location	Cytoplasm, Cell membrane
Synonyms	p164 ROCK 2; p164 ROCK-2; Rho associated coiled coil containing protein kinase 2; Rho associated protein kinase 2; Rho kinase 2; ROCK 2; Rock II; Rock2m; ROK alpha; ROKalpha; coiled-coil-containing protein kinase 2; KIAA0619; Rho associated, coiled coil containing protein kinase II; Rho-associated; Rho-associated protein kinase 2; Rock2; ROCK2_HUMAN.
Background	The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq, Jul 2008].
Application Dilution
WB	=1:500-1000