phospho-Histone H3 (Ser10) monoclonal Antibody

$Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:2000\r\nPrimary Ab incubation condition: 2 hours at\r\nroom temperature\r\nSecondary Ab: Goat Anti-Mouse IgG H&L\r\n(HRP)\r\nLysate: (-) HeLa, (+) HeLa+ Nocodazole\r\n(100ng\/ml, 18hr) + Calyculin A (100nM, 1hr)\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 17 kDa\r\nObserved MW: 17 kDa$

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:2000\r\nPrimary Ab incubation condition: 2 hours at\r\nroom temperature\r\nSecondary Ab: Goat Anti-Mouse IgG H&L\r\n(HRP)\r\nLysate: (-) HeLa, (+) HeLa+ Nocodazole\r\n(100ng\/ml, 18hr) + Calyculin A (100nM, 1hr)\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 17 kDa\r\nObserved MW: 17 kDa

Applications

WB
IHC-P
IHC-F
IF

Reactivity

Human

Predicted Reactivity

Mouse
Rat
Cow
Pig
Rabbit
Firefly

Overview
Catalog #	bsm-60078m
Product Name	phospho-Histone H3 (Ser10) monoclonal Antibody
Applications	WB IHC-P IHC-F IF
Reactivity	Human
Predicted Reactivity	Mouse, Rat, Cow, Pig, Rabbit, Firefly
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	KLH conjugated synthesised phosphopeptide derived from human Histone H3 around the phosphorylation site of Ser10
Clonality	Monoclonal
Clone #	A1F11
Isotype	IgG
Concentration	Lot Dependent
Purification	Purified by Protein G.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	8350
Swiss Prot	P68431
Subcellular location	Nucleus
Synonyms	Histone H3 (phospho S10); Phospho-Histone H3(Ser10); Histone H3(Phospho-Ser10); Histone H3(p-Ser10); Histone H3(Phospho-S10); H3S10ph; PHH3; H3 histone family member E pseudogene; H3F3; HIST3H3; Histone H3 3 pseudogene; H31_HUMAN; Histone H3; H3S; Histone H3-I/H3-II; Major histone H3; H3F; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l.
Background	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
Application Dilution
WB	=1:1000-2000
IHC-P	IHC-P=1:100-500
IHC-F	IHC-F=1:400-800
IF	IF=1:100-500