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Phospho-Histone H3 (Ser10) Monoclonal Antibody

HeLa (H) treated with Calyculin A (100nM) for 30 min, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with phospho-Histone H3 (Ser10) monoclonal antibody, unconjugated (bsm-60080M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
HeLa (H) treated with Calyculin A (100nM) for 30 min, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with phospho-Histone H3 (Ser10) monoclonal antibody, unconjugated (bsm-60080M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
NIH\/3T3 (M) + Serum (0%, overnight) add Nocodazole (100ng\/mL) for 18 h, add Calyculin A (100ng\/mL) for 1 h, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with phospho-Histone H3 (Ser10) monoclonal antibody, unconjugated (bsm-60080M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
NIH\/3T3 (M) + Serum (0%, overnight) add Nocodazole (100ng\/mL) for 18 h, add Calyculin A (100ng\/mL) for 1 h, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with phospho-Histone H3 (Ser10) monoclonal antibody, unconjugated (bsm-60080M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
C6 (R) treated with Calyculin A (100nM) for 30 min, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with phospho-Histone H3 (Ser10) monoclonal antibody, unconjugated (bsm-60080M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
C6 (R) treated with Calyculin A (100nM) for 30 min, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with phospho-Histone H3 (Ser10) monoclonal antibody, unconjugated (bsm-60080M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Histone H3 (Ser10) Monoclonal Antibody, Unconjugated (bsm-60080M) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Histone H3 (Ser10) Monoclonal Antibody, Unconjugated (bsm-60080M) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Histone H3 (Ser10) Monoclonal Antibody, Unconjugated (bsm-60080M) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Histone H3 (Ser10) Monoclonal Antibody, Unconjugated (bsm-60080M) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Histone H3 (Ser10) Monoclonal Antibody, Unconjugated (bsm-60080M) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Histone H3 (Ser10) Monoclonal Antibody, Unconjugated (bsm-60080M) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-40296G-HRP and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF
  • ICC/IF

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-60080m
Product Name Phospho-Histone H3 (Ser10) Monoclonal Antibody
Applications
WB IHC-P IHC-F IF ICC/IF
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Mouse
Clonality Monoclonal
Clone # C10C6
Isotype IgG1/kappa
Concentration Lot Dependent
Purification Protein G.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Swiss Prot P68431
Application Dilution
WB =1:500-1:1000
IHC-P IHC-P=1:100-500
IHC-F ICC/IF=1:50-1:100
IF
ICC/IF IF=0

Frequently asked questions

Which applications is Phospho-Histone H3 (Ser10) Monoclonal Antibody (bsm-60080M) validated for, and at what dilution?

bsm-60080M is validated for WB (WB=1:500-1:1000); IHC-P (IHC-P=1:100-500); IHC-F (ICC/IF=1:50-1:100); IF; ICC/IF (IF=0). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Phospho-Histone H3 (Ser10) Monoclonal Antibody (bsm-60080M) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-60080M be stored?

Store at -20°C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

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