Mono-Methyl-Histone H3 (Lys4) Monoclonal Antibody

Name: Mono-Methyl-Histone H3 (Lys4) Monoclonal Antibody
Brand: Bioss
SKU: bsm-60093M
Price: 490.0 USD
Availability: InStock

$Tissue: Rat testis\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60093M$

Tissue: Rat testis\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60093M

$Chromatin immunoprecipitation was performed using cross-linked chromatin from 5\u00d710\u2076 HeLa cells per IP, with 6 \u03bcg of bsm-60093M antibody or 6 \u03bcg of normal mouse IgG(negative control bs-0296P) , and 20 \u03bcL of Protein A\/G MagBeads per IP. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human GAPDH-CDS regions. The data are presented as enrichment of each sample relative to the total amount of input at each amplicon.$

Chromatin immunoprecipitation was performed using cross-linked chromatin from 5\u00d710\u2076 HeLa cells per IP, with 6 \u03bcg of bsm-60093M antibody or 6 \u03bcg of normal mouse IgG(negative control bs-0296P) , and 20 \u03bcL of Protein A\/G MagBeads per IP. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human GAPDH-CDS regions. The data are presented as enrichment of each sample relative to the total amount of input at each amplicon.

$25 ug total protein per lane of various lysates (see on figure) probed with Mono-Methyl-Histone H3 (Lys4) monoclonal antibody, unconjugated (bsm-60093M) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with Mono-Methyl-Histone H3 (Lys4) monoclonal antibody, unconjugated (bsm-60093M) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

$Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Mono-Methyl-Histone H3 (Lys4) Monoclonal Antibody, Unconjugated (bsm-60093M) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Mono-Methyl-Histone H3 (Lys4) Monoclonal Antibody, Unconjugated (bsm-60093M) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Mono-Methyl-Histone H3 (Lys4) Monoclonal Antibody, Unconjugated (bsm-60093M) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Mono-Methyl-Histone H3 (Lys4) Monoclonal Antibody, Unconjugated (bsm-60093M) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Mono-Methyl-Histone H3 (Lys4) Monoclonal Antibody, Unconjugated (bsm-60093M) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Mono-Methyl-Histone H3 (Lys4) Monoclonal Antibody, Unconjugated (bsm-60093M) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Mono-Methyl-Histone H3 (Lys4) Monoclonal Antibody, Unconjugated (bsm-60093M) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Mono-Methyl-Histone H3 (Lys4) Monoclonal Antibody, Unconjugated (bsm-60093M) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Mono-Methyl-Histone H3 (Lys4) Monoclonal Antibody, Unconjugated (bsm-60093M) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Mono-Methyl-Histone H3 (Lys4) Monoclonal Antibody, Unconjugated (bsm-60093M) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Mono-Methyl-Histone H3 (Lys4) Monoclonal Antibody, Unconjugated (bsm-60093M) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Mono-Methyl-Histone H3 (Lys4) Monoclonal Antibody, Unconjugated (bsm-60093M) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Applications

WB
IHC-F
IF
IHC-P

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-60093m-100uL
Product Name	Mono-Methyl-Histone H3 (Lys4) Monoclonal Antibody
Applications	WB IHC-F IF IHC-P
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Mouse
Clonality	Monoclonal
Clone #	G4G10
Isotype	IgG2B/kappa
Concentration	Lot Dependent
Purification	Protein G.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Swiss Prot	P68431
Application Dilution
WB
IHC-F
IF
IHC-P

Recombinant Proteins (4).png__PID:b4703663-ad26-4eba-90ef-48f549942e61

bsm-60093m-100uL Copy

Data Sheet